Articles by Miladys Limonta Fernández

Articles by Miladys Limonta Fern?ndez

Miladys Limonta Fernández

miladys.limonta@cigb.edu.cu.

Principal Researcher at the Centre for Genetic Engineering and Biotechnology

Articles

Scale up of Fed-Batch Culture to Produce Plasmid DNA in Escherichia coli (Peer Reviewed)
July 1, 2012
By: Odalys Ruiz Hernández , Jorge Valdes Hernández , Willy Frometa Planche , Michel Diaz Martínez , Daniel Alvares Almiñaque , Marta Pupo Peña , Miladys Limonta Fernández , Dinorah Torres Idahody, PhD , Eduardo Martínez, PhD

The authors present scale-up from a 5-L fermentor to a 50-L pilot-scale using the criterion of constant power consumption per unit liquid volume.

The Purification of Plasmid DNA for Clinical Trials Using Membrane Chromatography
February 1, 2010
By: Miladys Limonta Fernández , Gabriel Márquez , Martha Pupo , Odalys Ruiz Hernández

Membrane chromatography ensures purity at high flow rates.

High-Cell-Density Culture to Produce Plasmid DNA for Gene Therapy in E. coli
July 1, 2009
By: Odalys Ruiz Hernández , Mariela Pérez de la Iglesia , Martha Pupo , Miladys Limonta Fernández , Dinorah Torres Idahody, PhD , Saily Martínez Gómez , Karelia Macias Cosme , Yanay Proenza Jimenez , Jorge Valdes Hernández , Eduardo Martínez, PhD

How to produce Plasmid DNA in a high-cell-density culture.

Plasmid DNA Recovery Using Size-Exclusion and Perfusion Chromatography
September 1, 2008
By: Miladys Limonta Fernández , Gabriel Márquez , Isabel Rey , Martha Pupo , Odalys Ruiz Hernández , Yalena Amador-Canizares , Santiago Duenas-Carrera

Scalable method to recover plasmid-DNA.