This application note highlights the performance characteristics of both the 200Å and 450Å pore-size versions of Waters new XBridge Protein BEH SEC Columns, designed for the separation of macromolecular proteins, with respect to resolution, column-to-column reproducibility, and column stability.
The first PEGylated biotherapeutic, pegademase, which is a bioconjugate of the bovine derived enzyme adenosine deaminase and 5 KDa molecular weight (MW) polyethylene glycol (PEG), was introduced in 1990. Pegademase is used for the treatment of individuals with severe combined immunodeficiency disease (SCID).
In this study, we evaluate Charged Surface Hybrid (CSH) C18 column chemistry for peptide separations, expecting similar improvements in chromatographic performance since most peptides under commonly employed acidic conditions contain a positive charge. This work with peptides demonstrates that this novel stationary phase exhibits greater peak capacity, unique selectivity, and less dependence on mass spectrometry signal suppression, strong ion pairing agents in peptide analysis.
Glycan characterization has remained a challenging aspect of biotherapeutic characterization compared to techniques such as intact mass or peptide map analysis, which most labs consider routine today. The addition of the glycan UPLC/FLR/MS workflow and use of the experimentally derived Waters Glycan GU Library within the Glycan Application Solution with UNIFI have addressed the desire for compliant-ready, automated, high-confidence glycan structure assignments by enabling rapid acquisition, review, and communication of individual glycan profile results, and the larger sets of glycan analyses used for comparability studies.
The analysis of natural and synthetic opioid drugs continues to be an important aspect of forensic toxicology. A substantial percentage of arrests and/or deaths are attributed to the misuse or abuse of narcotic pain relievers such as oxycodone and hydrocodone, as well as the illegal opiate, heroin.
The robustness and reliability of pharmacokinetic (PK) data is an essential part of bioanalysis. LC-MS is the technique of choice in quantitative bioanalysis due to the high selectivity and sensitivity the technique offers.