Articles by Alahari Arunakumari, PhD - BioPharm International

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Articles by Alahari Arunakumari, PhD

Alahari Arunakumari, PhD


Senior Director of Process Development at Medarex

Articles
Precipitation of Process-Derived Impurities in Non-Protein A Purification Schemes for Antibodies
October 2, 2009

Precipitation prior to capture chromatography offers a simple, robust, and economical method to remove CHO host cell proteins and DNA.

The Impact of Cell Culture Medium on Cell Line and Process Development Timelines and Strategies
June 2, 2009

ACF medium sped up cell-line development.

Advances in Non-Protein A Purification Processes for Human Monoclonal Antibodies
March 2, 2009

In three non-affinity purification processes based on cation exchange capture with high binding capacity, applying a host cell protein exclusion strategy enabled robust scale up and better economics.

Optimizing the Primary Recovery Step in Nonaffinity Purification Schemes for HuMAbs
March 2, 2008

An alternative approach to traditional Protein A schemes is comparable in overall efficiency, product recovery, and quality.

Chromatography: A Two-Column Process To Purify Antibodies Without Protein A
May 1, 2007

This flexible setup minimizes the number of purification process steps, buffers, and process components.

ALTERNATIVES TO PROTEIN A: Improved Downstream Process Design for Human Monoclonal Antibody Production
February 2, 2007

Affinity purification schemes for antibody production have certain limitations keeping up with cell culture expression levels as they reach and exceed 10 g/L. New downstream purification processes are based on low cost, long lasting, and high binding (40–100 mg/mL) cation exchange resins.

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