Tom Wheat, Ph.D.

Throughout the development of a biopharmaceutical protein, peptide mapping is used to demonstrate genetic stability and to confirm the intergrity of the protein. This whitepaper focuses on the quantitative aspects of UPLC peptide mapping with UV detection.

Ultra performance liquid chromatography (UPLC) is a new category of liquid chromatography that researchers are using to increase resolution, speed, and sensitivity in a variety of applications. These benefits result from packing columns with 1.7 ?m particles and using instruments that are optimized for such columns.