Articles by Agilent Technologies - BioPharm International

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Articles by Agilent Technologies

Does Tween 20 Affect Monoclonal Antibody Separation?

Oct 29, 2013

Tween 20 has various beneficial properties for biological analysis, such as stabilizing proteins, solubilizing protein membranes, and preventing nonspecific binding of primary and secondary antibodies. Therefore, Tween 20 is often present in samples of monoclonal antibodies that require analysis using techniques such as SEC. This app note investigates whether the presence of Tween 20 detergent directly in the sample and in the eluent during multiple injections of an IgG sample onto an Agilent Bio SEC column affects the analysis or the columns.

N-Terminal Site-Specific PEGylation and Analytical-Scale Purification of PEG Lysozyme

Oct 29, 2013

Analytical-scale purification of PEG lysozyme was developed using an Agilent Bio-LC and Poroshell column. PEGylated lysozymes were re-analyzed by SEC and RP HPLC. The results indicated that purified PEG lysozyme was homogenous.

Precise Determination of Protein Molecular Weight

Sep 24, 2013

This Application Note shows the determination of molecular weight, size, and relative concentration of protein samples after SEC with the Agilent 1260 Infinity Bio-inert Quaternary LC System with Multi-Detector GPC/SEC.

Protein Separation with pH Gradients Using Composite Buffer Systems

Apr 30, 2013

Tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range using composite buffer systems.

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