Articles by Agilent Technologies - BioPharm International

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Articles by Agilent Technologies

Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures

Mar 25, 2014

To monitor monoclonal antibody titer and yield from cell-culture supernatants before expensive prep and large amounts of Protein A are employed, an analytical scale procedure helps determine the titer of monoclonal antibody for the optimal time for harvest of the mAb product. Here, pre-packed Agilent Bio-Monolith Protein A columns quickly capture mAb titer from cell supernatant.

Fast and Efficient Peptide Mapping of a Monoclonal Antibody (mAb): UHPLC Performance Using Superficially Porous Particles

Mar 25, 2014

In this work, an AdvanceBio Peptide Mapping column is used to generate a rapid and highly efficient peptide map at a traditional LC system pressure. The column achieves substantial improvements in peptide mapping during very fast run times and low system pressures, while still maintaining high peak-performance efficiency.

Peptide Mapping of Glycoprotein Erythropoietin by HILIC LC/MS and RP-LC/MS

Mar 25, 2014

In this application note an Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300-HILIC 1.8 ?m column and an Agilent AdvanceBio Peptide Mapping column are used in combination with time of flight (TOF) mass-spectrometry (MS) for mapping EPO protein. The work demonstrates the utility of HILIC as an orthogonal and complementary approach to reversed-phase LC/MS for peptide analysis.

Charge Variant Analysis using pH Gradients

Jan 28, 2014

This Application Note shows method development for charge variant profiling of monoclonal antibodies using pH gradient elution with Agilent Buffer Advisor Software and online pH and conductivity monitoring.

Affinity Removal High Abundant Proteins in Human Plasma

Dec 27, 2013

This Application Note describes the depletion of high abundant proteins from plasma using the Agilent Multiple Affinity Removal System coupled with the Agilent 1260 Infinity Bio-inert Quaternary LC.

Analysis of Monoclonal Antibody Digests using 2D-LC

Nov 26, 2013

This Application Note demonstrates the potential of comprehensive two-dimensional liquid chromatography using the Agilent 1290 Infinity 2D-LC Solution with DAD detection for the analysis of monoclonal antibody tryptic digests.

N-Terminal Site-Specific PEGylation and Analytical-Scale Purification of PEG Lysozyme

Nov 26, 2013

In this study, hen egg white lysozyme was used as a model protein for PEGylation using N-terminal specific mPEG propionaldehyde (PEG aldehyde) in presence of cyanoborohydrate. A method for the analytical-scale purification of PEG lysozyme was developed using an Agilent 1260 Infinity Bio-Inert LC System and an Agilent Poroshell 120 SB-C18 column. Analytical-scale Fraction Collector with peak-based fraction collection was employed to collect the purified PEG lysozyme. The fractions were then re-analyzed by RP HPLC and SEC to demonstrate the homogeneity of the purified PEG conjugate.

Does Tween 20 Affect Monoclonal Antibody Separation?

Oct 29, 2013

Tween 20 has various beneficial properties for biological analysis, such as stabilizing proteins, solubilizing protein membranes, and preventing nonspecific binding of primary and secondary antibodies. Therefore, Tween 20 is often present in samples of monoclonal antibodies that require analysis using techniques such as SEC. This app note investigates whether the presence of Tween 20 detergent directly in the sample and in the eluent during multiple injections of an IgG sample onto an Agilent Bio SEC column affects the analysis or the columns.

N-Terminal Site-Specific PEGylation and Analytical-Scale Purification of PEG Lysozyme

Oct 29, 2013

Analytical-scale purification of PEG lysozyme was developed using an Agilent Bio-LC and Poroshell column. PEGylated lysozymes were re-analyzed by SEC and RP HPLC. The results indicated that purified PEG lysozyme was homogenous.

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