Precipitation prior to capture chromatography offers a simple, robust, and economical method to remove CHO host cell proteins and DNA.
Oct 2, 2009
By:
Jue (Michelle) Wang, PhD, Timothy Diehl, Deena Aguiar, Xiao-Ping Dai, PhD, Alahari Arunakumari, PhD
Precipitation prior to capture chromatography offers a simple, robust, and economical method to remove CHO host cell proteins and DNA.
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In three non-affinity purification processes based on cation exchange capture with high binding capacity, applying a host cell protein exclusion strategy enabled robust scale up and better economics.
Mar 2, 2009
By:
Alahari Arunakumari, PhD, Jue (Michelle) Wang, PhD, Gisela Ferreira, PhD
In three non-affinity purification processes based on cation exchange capture with high binding capacity, applying a host cell protein exclusion strategy enabled robust scale up and better economics.
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An alternative approach to traditional Protein A schemes is comparable in overall process efficiency, product recovery, and quality.
Mar 2, 2008
By:
Jue (Michelle) Wang, PhD, Timothy Diehl, Mark Watkins-Fischl, Deborah Perkins, Deena Aguiar, Alahari Arunakumari, PhD
An alternative approach to traditional Protein A schemes is comparable in overall efficiency, product recovery, and quality.
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A two-step process—a cation exchange capture column followed by anion exchange flow-through membrane chromatography—resulted in >80% process yield, higher batch capacity, and shorter process time.
Feb 2, 2007
By:
Alahari Arunakumari, PhD, Jue (Michelle) Wang, PhD, Gisela Ferreira
Affinity purification schemes for antibody production have certain limitations keeping up with cell culture expression levels as they reach and exceed 10 g/L. New downstream purification processes are based on low cost, long lasting, and high binding (40–100 mg/mL) cation exchange resins.
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