Articles by Massimo Morbidelli, PhD - BioPharm International

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Articles by Massimo Morbidelli, PhD

Increasing Capacity Utilization in Protein A Chromatography

An mAb purification study tests a twin-column capture process.
Oct 1, 2013

A twin-column periodic countercurrent capture process that combines an efficient sequential, countercurrent loading process with a minimal twin-column hardware configuration is described.

Protein Peptide Purification using the Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process

A new method for MAb purification.
Jan 1, 2009

A new method for MAb purification.

CONTINUOUS PROCESSING: The Multicolumn Countercurrent Solvent Gradient Purification Process

A continuous chromatographic process for monoclonal antibodies without using Protein A
Feb 2, 2007

This article presents the multicolumn countercurrent solvent gradient purification (MCSGP) process, which uses three chromatographic columns, and incorporates the principle of countercurrent operation and the possibility of using solvent gradients. A MCSGP prototype has been built using commercial chromatographic equipment. The application of this prototype for purifying a MAb from a clarified cell culture supernatant using only a commercial, preparative cation exchange resin shows that the MCSGP process can result in purities and yields comparable to those of purification using Protein A.

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