Using on-line HPLC to monitor the eluent from process-scale columns allows the process decision—when to start and stop collection—to be based on a critical quality attribute rather than on a surrogate measurement.
Feb 2, 2007
This article discusses how on-line high-performance liquid chromatography (HPLC) can measure product purity in the column eluent stream in near–real time. These data can then enable the automation and control of a purification column operation, thus reducing product variability, shortening process cycle time, and increasing yield. An example application demonstrates how on-line HPLC is used as a process analytical technology to ensure the process can accommodate variability in the separation while ensuring the product meets its critical quality attributes.