Articles by Alahari Arunakumari, PhD - BioPharm International

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Articles by Alahari Arunakumari, PhD

Precipitation of Process-Derived Impurities in Non-Protein A Purification Schemes for Antibodies

Precipitation prior to capture chromatography offers a simple, robust, and economical method to remove CHO host cell proteins and DNA.
Oct 2, 2009

Precipitation prior to capture chromatography offers a simple, robust, and economical method to remove CHO host cell proteins and DNA.

The Impact of Cell Culture Medium on Cell Line and Process Development Timelines and Strategies

Animal-component free (ACF) medium sped up cell-line development by eliminating the adaptation period from serum-containing medium used in early development to ACF medium used for high-titer production.
Jun 2, 2009

ACF medium sped up cell-line development.

Advances in Non-Protein A Purification Processes for Human Monoclonal Antibodies

In three non-affinity purification processes based on cation exchange capture with high binding capacity, applying a host cell protein exclusion strategy enabled robust scale up and better economics.
Mar 2, 2009

In three non-affinity purification processes based on cation exchange capture with high binding capacity, applying a host cell protein exclusion strategy enabled robust scale up and better economics.

Optimizing the Primary Recovery Step in Nonaffinity Purification Schemes for HuMAbs

An alternative approach to traditional Protein A schemes is comparable in overall process efficiency, product recovery, and quality.
Mar 2, 2008

An alternative approach to traditional Protein A schemes is comparable in overall efficiency, product recovery, and quality.

Chromatography: A Two-Column Process To Purify Antibodies Without Protein A

May 1, 2007

This flexible setup minimizes the number of purification process steps, buffers, and process components.

ALTERNATIVES TO PROTEIN A: Improved Downstream Process Design for Human Monoclonal Antibody Production

A two-step process—a cation exchange capture column followed by anion exchange flow-through membrane chromatography—resulted in >80% process yield, higher batch capacity, and shorter process time.
Feb 2, 2007

Affinity purification schemes for antibody production have certain limitations keeping up with cell culture expression levels as they reach and exceed 10 g/L. New downstream purification processes are based on low cost, long lasting, and high binding (40–100 mg/mL) cation exchange resins.

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