Articles by Hank Kopf - BioPharm International

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Articles by Hank Kopf

Development of a Novel Platform TFF System for Insect

Development of a Novel Platform TFF System for Insect Cell Culture Harvest A single-membrane system was used to clarify insect cells, diafilter the cell concentrates, mix with an elution buffer to release the target protein, and collect the released target protein in the filtrate. The one tank–one module method simplifies and improves the harvest in comparison to multiple centrifugation and filtration steps.
Jun 2, 2006

The overall average flux rate for the concentration and diafiltration step was 41 L m–2 h–1.

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