Taken together, these data demonstrate that expansion of hMSCs in 3D culture demonstrate similar flow cytometry profiles,
differentiation ability, and functional status as hMSCs expanded in 2D culture. After two weeks, cells reach densities greater
than 2×105 cells/mL while maintaining their identity as shown by the surface expression of CD105, CD90, and CD73 and the absence of
CD14, CD34, and CD45.
The production of well-characterized hMSCs in stirred-tank bioreactors using microcarriers is the first step towards establishing
a scalable, single-use production process for the large-scale industrialization of stem cells. The Mobius CellReady 3-L bioreactor
presents several advantages compared with multilayer flatbed cultures, including lower overall cost, ease of use, and the
ability to closely monitor process parameters through-out expansion and make adjustments while cells are still viable, if
Donghui Jing, PhD*, is a research scientist III, Stem Cell Initiative at EMD Millipore; Neethu Sunil is a research scientist at Experis, Southborough, Mass. Sandhya Punreddy is a research scientist III; Manjula Aysola, PhD, is a research scientist III; Daniel Kehoe, PhD, is a research scientist III; Julie Murrel, PhD, is a senior scientist; Martha Rook, PhD, is head of upstream and sensor technologies; and Knut Niss, PhD, is R&D manager; all at EMD Millipore, Bedford, Mass.
*To whom correspondence should be addressed, email@example.com
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