Evaluation of Single-Use Fluidized Bed Centrifuge System for Mammalian Cell Harvesting - This article discusses the evaluation of a novel single-use fluidized bed centrifuge for harvesting of antibodi

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Evaluation of Single-Use Fluidized Bed Centrifuge System for Mammalian Cell Harvesting
This article discusses the evaluation of a novel single-use fluidized bed centrifuge for harvesting of antibodies.


BioPharm International
Volume 25, Issue 11, pp. 34-40

CONCLUSION

The single-use FBC was evaluated for cell-harvesting applications. After determining the maximum capacity of each single-use chamber and the initial and processing flow rates, five total cell-harvesting runs were completed. Cell density, NTU, LDH levels, and residual DNA content from the centrate were collected for evaluating the system. Low cell density and turbidity in the centrate indicated that clarification efficiency was high, and little to no change in the LDH level tle cell lysis during processing. Compared to disc-stack centrifuges and other current standard technologies, it can be concluded that the FBC system is an attractive single-use alternative to current options for cell harvesting.

ACKNOWLEDGMENTS

The authors would like to acknowledge the following individuals for their contributions: Sunil Mehta and Tod Herman for providing their expertise on the FBC; Divya Harjani and Nikhil Patel for maintaining CHO cell cultures and assistance in carrying out some of the FBC runs; Meredith Rice for her assistance with the turbidimeter; and the Janssen R&D Process Analytical Support group for analyzing the residual DNA and antibody titer samples.

Hsu-Feng Ko is a research scientist and Ravi Bhatia is associate director, both at Janssen Research & Development, Spring House, PA.

REFERENCES

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