POLYBIND–Z PRODUCTION COSTS AND DISPOSABLE USE
One of the value propositions we seek to develop is the overall cost reduction of using PolyBind–Z beads in antibody capture.
It is estimated that 60%–80% of the buffer cost comes from cleaning of the purification resins. These costs are eliminated
if disposable media are incorporated into the process. For comparison we modelled the PolyBind–Z as a single use resin in
a cross-flow membrane system versus traditional packed bed methods (see Table II). What is not reflected in the relative costs
is the cost of buffer preparation and storage. In the interest of consistency, we have only compared consumables and not the
Table II: Projected relative cost savings: PolyBind–Z used as a single use resin in a cross-flow membrane system versus a
leading Protein A sepharose used in a traditional packed bed column system.
We assume the cost of buffers and time saved in regeneration, sterilization, and validation represent the primary cost savings
in the process. Fraud et al presented data comparing ion exchange resin chromatography to membrane adsorber chromatography
(13). In their case study, they demonstrated that up to 95% of the buffer and water used for cleaning and sterilization are
saved in disposable systems. Combined with other factors, such as smaller footprint for buffer storage, and shorter process
times due to convective versus diffusive binding, the argument for shifting to a disposable system is compelling.
Bernd Rehm, PhD,* is CSO, Tracy Thompson is CEO and Matthew Plassmeyer, PhD, is project manager, R&D, all at PolyBatics, Palmerston North, New Zealand. B.Rehm@massey.ac.nz
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