Single-use bioreactors have become accepted by industry and academic laboratories because of ease of use, flexibility, cost
of operation, and lower investments. The CELL-tainer single-use bioreactor system can be used in a wide variety of applications:
intensive cell cultures, fragile cultivation systems like with micro-carriers and hybridoma cultures, and also in microbial
fermentations (e.g. yeast and fungal cultures) due to its ability to deliver proper mixing and high mass transfer. Because
the system is equipped with advanced process control, which could include in-line analysis of glucose and lactate, the CELL-tainer
widens the application of single-use bioreactors even to traditional biotechnology processes as a process development tool
or as pre-culture system.
The authors would like to thank Nick van Biezen and Jeroen Schouwenberg (HAN Biocenter, Nijmegen, The Netherlands) and Anton
Tromper (CELLution Biotech) for their contribution in the E.coli cultivation work.
Dr. Nico M.G. Oosterhuis is CTO/CSO at CeLLution Biotech, Assen, The Netherlands (email@example.com
). Hans J. van den Berg is an independent business developer.
1. V. Singh, Disposable bioreactor for cell culture using wave-induced agitation, Cytotechnology, 30: 149-158 (1999).
2. J. Simola, Characterization of WAVE Bioreactor to scale-up mAb cell culture process from 10L to 100L, Esact Conference
3. R. Eibl, D. Eibl. "Design and use of the Wave Bioreactor for plant cell culture," in Plant Tissue Culture Engineering,
G.S. Dutta and Y. Ibaraki, Eds. (Springer, New York, NY, 2006).
4. P. Van der Heiden, M. Buevink, N.M.G. Oosterhuis WO 2007/001173 A2 (2007).
5. L.L. Weng, Strategies for accelerated development of microbial fermentation processes (Boston, November 2009).