Systematic Approaches to Develop Chemically Defined Cell Culture Feed Media - It is important to ensure that the transition from peptone-containing to CD media doesn't affect product quality. - BioPha

ADVERTISEMENT

Systematic Approaches to Develop Chemically Defined Cell Culture Feed Media
It is important to ensure that the transition from peptone-containing to CD media doesn't affect product quality.


BioPharm International
Volume 23, Issue 11

CONCLUSION

Systematic feed medium development approaches were successfully used to enable the transition from a peptone-containing process to a process using chemically-defined basal and feed media. An iterative process was used to increase titers based on the metabolic needs of specific cell lines. In addition, we have successfully conducted filterability, medium preparation, and scale-up studies. These CD formulations have the potential to increase process robustness, generate higher titer processes with acceptable product quality, and be used for further medium optimization efforts.

ACKNOWLEGEMENTS

The authors would like to acknowledge the contributions of Gayle Derfus, Roberto Flores, Jennifer Autsen, Joana Caria, Dacia Brooks, Masaru Shiratori, and Robert Shawley for their guidance, ideas, and support.

Efren Pacis is a senior research associate, Jincai Li is a senior engineer, Ashraf Amanullah is a director, and Feng Li is a senior engineer, all at Oceanside Pharma Technical Development, Genentech, Inc. Oceanside, CA, 760.231.2127,
Natarajan Vijayasankaran is engineer II and Martin Gawlitzek is a senior scientist, both at late stage cell culture, US Biologics Pharma Technical Development, Genentech, Inc., South San Francisco, CA.

REFERENCES

1. Fike R. Nutrient supplementation strategies for biopharmaceutical production, Part 1: Identifying a nutrient supplementation formulation. BioProcess Int. 2009;7(10): 44–51.

2. Fike R. Nutrient supplementation strategies for biopharmaceutical production, Part 2 Feeding for optimal recombinant protein production. BioProcess Int. 2009;7(11):46–52.

3. Vijayasankaran N Li J, Shawley R, Chen A, Shiratori M, Gawlitzek M, Li F, Kiss R, Amanullah A. Animal cell culture media. In: Flickinger MC, ed. Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology. New York: John Wiley & Sons, Inc. 2010; p. 261–75.

4. Heidemann R, Zhang C, Qi H. Larrick Rule J, Rozales C, Park S, Chuppa S, Ray M, Michaels J, Konstantinov K, Naveh D. The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cells. Cytotechnol. 2000;32(2):157–67.

5. Merten, OW. Virus contaminations of cell cultures —A biotechnological view. Cytotechnol. 2002;39(2):91–116.

6. Grosvenor S. The role of media development in process optimization: an historical perspective. BioPharm Int Suppl. Guide to Protein Production. 2008 Jun: pp 28–36.

7. Hodge G. Media development for cell Culture. Biopharm Int. 2005;18(5):54–57 .

8. Arunakumari A, Dai X, Goldstein J, Kloth C, Ghebremariam H, MacIsaac G, Wagner M. The impact of cell culture medium on cell line and process development and timelines and strategies. BioPharm Int Suppl. Advances in Fermentation and Cell Culture: the keys to upstream productivity; 2009 June: pp. 4–15.

9. Ishaque A, Al-Rubeai M. Role of vitamins in determining apoptosis and extent of suppression by bcl-2 during hybridoma cell culture. Apoptosis 2002;7(3):231–9.

10. Ducommun P, Ruffieux PA, von Stockar U, Marison I. The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production. Cytotechnol. 2001;37(2):65–73.


blog comments powered by Disqus

ADVERTISEMENT

ADVERTISEMENT

Lilly to Acquire Novartis Animal Health
April 22, 2014
Lilly to Acquire Novartis Animal Health
April 22, 2014
Novartis and GSK Trade Assets
April 22, 2014
EMA Warns of Falsified Herceptin Vials
April 16, 2014
Mallinckrodt to Acquire Questcor Pharmaceuticals
April 16, 2014
Author Guidelines
Source: BioPharm International,
Click here