CONCLUSION
Systematic feed medium development approaches were successfully used to enable the transition from a peptone-containing process
to a process using chemically-defined basal and feed media. An iterative process was used to increase titers based on the
metabolic needs of specific cell lines. In addition, we have successfully conducted filterability, medium preparation, and
scale-up studies. These CD formulations have the potential to increase process robustness, generate higher titer processes
with acceptable product quality, and be used for further medium optimization efforts.
ACKNOWLEGEMENTS
The authors would like to acknowledge the contributions of Gayle Derfus, Roberto Flores, Jennifer Autsen, Joana Caria, Dacia
Brooks, Masaru Shiratori, and Robert Shawley for their guidance, ideas, and support.
Efren Pacis is a senior research associate, Jincai Li is a senior engineer, Ashraf Amanullah is a director, and Feng Li is a senior engineer, all at Oceanside Pharma Technical Development, Genentech, Inc. Oceanside, CA, 760.231.2127, fengl@gene.com
Natarajan Vijayasankaran is engineer II and Martin Gawlitzek is a senior scientist, both at late stage cell culture, US Biologics Pharma Technical Development, Genentech, Inc., South
San Francisco, CA.
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