Systematic Approaches to Develop Chemically Defined Cell Culture Feed Media - It is important to ensure that the transition from peptone-containing to CD media doesn't affect product quality. - BioPha

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Systematic Approaches to Develop Chemically Defined Cell Culture Feed Media
It is important to ensure that the transition from peptone-containing to CD media doesn't affect product quality.


BioPharm International
Volume 23, Issue 11

CONCLUSION

Systematic feed medium development approaches were successfully used to enable the transition from a peptone-containing process to a process using chemically-defined basal and feed media. An iterative process was used to increase titers based on the metabolic needs of specific cell lines. In addition, we have successfully conducted filterability, medium preparation, and scale-up studies. These CD formulations have the potential to increase process robustness, generate higher titer processes with acceptable product quality, and be used for further medium optimization efforts.

ACKNOWLEGEMENTS

The authors would like to acknowledge the contributions of Gayle Derfus, Roberto Flores, Jennifer Autsen, Joana Caria, Dacia Brooks, Masaru Shiratori, and Robert Shawley for their guidance, ideas, and support.

Efren Pacis is a senior research associate, Jincai Li is a senior engineer, Ashraf Amanullah is a director, and Feng Li is a senior engineer, all at Oceanside Pharma Technical Development, Genentech, Inc. Oceanside, CA, 760.231.2127,
Natarajan Vijayasankaran is engineer II and Martin Gawlitzek is a senior scientist, both at late stage cell culture, US Biologics Pharma Technical Development, Genentech, Inc., South San Francisco, CA.

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