The Effect of Limiting Phosphate Using the HMS174 Cell Line - Surprising results from a case study using the HMS174 cell line. - BioPharm International

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The Effect of Limiting Phosphate Using the HMS174 Cell Line
Surprising results from a case study using the HMS174 cell line.


BioPharm International
Volume 23, Issue 7

ACKNOWLEDGMENTS

This work was supported by Grant 39129 from the Bill & Melinda Gates Foundation.

Garner G. Moulton is a senior research associate and Thomas Vedvick is director of process sciences, both in the process sciences department at the Infectious Disease Research Institute, Seattle, WA, 206.330.2549,
.

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3. Mare L, Cimander C, Elfwing A, Hagander P. Feeding strategies for e. coli fermentations demanding an enriched environment. Bioprocess Biosyst Eng. 2006;30:13–25.

4. Shinar G, Rabinowitz J, Alon U. Robustness in Glyoxylate Bypass Regulation. PloS Comput Biol. 2009;5(3):1–8.

5. Saier M. Bacterial phosphoenolpyruvate: sugar phosphatransferase systems: structural, functional, and evolutionary interrelationships. Bacteriol Rev. 1977;41(4):856–71.

6. Sooan S, Chang D, Pan J. Acetate consumption activity directly determines the level of acetate accumulation during escherichia coli W3110 growth. J Microbiol Biotechnol. 2009;19(10):1127–34.

7. Wolfe A. The acetate switch. Microbiol Mol Biol Rev. 2005;69(1):12–50.

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9. Seaver L, Imlay J. Hydrogen peroxide fluxes and compartmentatlization inside frowing escherichia coli. 2001;183(24):7182–9.

10. Eiteman M, Altman E. Overcoming acetate in escherichia coli recombinant protein fermentations. Trends in Biotechnol. 2006;24(11).

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