The Effect of Limiting Phosphate Using the HMS174 Cell Line - Surprising results from a case study using the HMS174 cell line. - BioPharm International

ADVERTISEMENT

The Effect of Limiting Phosphate Using the HMS174 Cell Line
Surprising results from a case study using the HMS174 cell line.


BioPharm International
Volume 23, Issue 7

ACKNOWLEDGMENTS

This work was supported by Grant 39129 from the Bill & Melinda Gates Foundation.

Garner G. Moulton is a senior research associate and Thomas Vedvick is director of process sciences, both in the process sciences department at the Infectious Disease Research Institute, Seattle, WA, 206.330.2549,
.

REFERENCES

1. Farmer W, Liao J. Reduction of aerobic acetate production by escherichia coli. Appl Env Microbiol. 1997; 63(8):3205–10.

2. Lin H, Castro N, Bennet G, San K. Acetyl-CoA synthetase overexpression in escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering. Appl Microbiol Biotechnol. 2006;71:870–4.

3. Mare L, Cimander C, Elfwing A, Hagander P. Feeding strategies for e. coli fermentations demanding an enriched environment. Bioprocess Biosyst Eng. 2006;30:13–25.

4. Shinar G, Rabinowitz J, Alon U. Robustness in Glyoxylate Bypass Regulation. PloS Comput Biol. 2009;5(3):1–8.

5. Saier M. Bacterial phosphoenolpyruvate: sugar phosphatransferase systems: structural, functional, and evolutionary interrelationships. Bacteriol Rev. 1977;41(4):856–71.

6. Sooan S, Chang D, Pan J. Acetate consumption activity directly determines the level of acetate accumulation during escherichia coli W3110 growth. J Microbiol Biotechnol. 2009;19(10):1127–34.

7. Wolfe A. The acetate switch. Microbiol Mol Biol Rev. 2005;69(1):12–50.

8. Kleman G, Strohl W. Acetate metabolism by escherichia coli in high-cell-density fermentation. App Env Microbiol. 1994;60(11):3952–8.

9. Seaver L, Imlay J. Hydrogen peroxide fluxes and compartmentatlization inside frowing escherichia coli. 2001;183(24):7182–9.

10. Eiteman M, Altman E. Overcoming acetate in escherichia coli recombinant protein fermentations. Trends in Biotechnol. 2006;24(11).

11. Cozzone A. Regulation of Acetate Metabolism by Protein Phosphorylation in Enteric Bateria. Annu Rev Microbiol. 1998;52:127–64.


blog comments powered by Disqus

ADVERTISEMENT

ADVERTISEMENT

FDA Panel Unanimously Backs Secukinumab for the Treatment of Psoriasis
October 22, 2014
Roche to Expand and Improve its Basel Site
October 22, 2014
Pall ForteBio Releases Bioprocessing Contamination Detection Kit
October 22, 2014
EMA Works to Speed Up Ebola Treatment
October 20, 2014
Amgen Sues Sanofi and Regeneron over Patent for mAb Targeting PCSK9
October 20, 2014
Author Guidelines
Source: BioPharm International,
Click here