Using Information Technology to Enhance Upstream Productivity - - BioPharm International


Using Information Technology to Enhance Upstream Productivity

BioPharm International Supplements


The authors would like to thank Christian Kaiser from Richter-Helm Biologics, Hamburg, Germany, and Babatunde A. Ogunnaike, University of Delaware for their support and for the detailed insight into their research.

KARL RIX, PHD, is the director of North American operations and MARTIN GROLMS is a technical editor in marketing and communications, both at DASGIP AG, Jülich, Germany, +49 (0)2461 980 121,


1. Eiteman MA, Altman E. Overcoming acetate in Escherichia coli recombinant protein fermentations, Trends Biotechnol. 2006;24(11):530–6.

2. Sorensen H, Mortensen K. (2005) Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli, Microbial Cell Factories, 4, Available from:

3. Ramirez DM, Bentley WE. Fed-batch feeding and induction policies that improve foreign protein synthesis and stability by avoiding stress response. Biotechnol Bioeng. 1995;47:596–608.

4. Strandberg L, Enfors S-O. Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli. Appl Env Microbiol. 1991;57(6):1669–74.

5. Choi JH, Keum KC, Lee SY. Production of recombinant proteins by high cell density cultures of Escherichia coli, Chem Eng Sci. 2006;61:876–85.

6. Borys MC, Linzer DIH, Papoutsakis ET. Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-1 by Chinese hamster ovary cells in a pH-dependent manner. Biotechnol Bioeng. 1994;43:505–14.

7. Gawlitzek M, Estacio M, Furch T, Kiss R. Identification of Cell Culture Conditions to Control N-Glycosylation Site-Occupancy of Recombinant Glycoproteins Expressed in CHO Cells. Biotechnology and Bioengineering. 2009;103:1164–75.

8. Hayter PM, Curling EMA, Baines AJ, Jenkins N, Salmon I, Strange PG, Tong JM, Bull AT. Glucose-limited chemostat culture of Chinese hamster ovary cells producing recombinant human interferon-gamma. Biotechnol Bioeng. 1992;39:327–35.

9. Kunkel JP, Jan DCH, Butler M, Jamieson JC. Comparisons of the glycosylation of a monoclonal antibody produced under nominally identical cell culture conditions in two different bioreactors. Biotechnol Progr. 2000;16:462–70.

10. Muthing J, Kemminer SE, Conradt HS, Sagi D, Nimtz M, Karst U, Peter-Katalinic J. Effects of buffering conditions and culture pH on production rates and glycosylation of clinical phase I anti-melanoma mouse IgG3 monoclonal antibody R24. Biotechnol Bioeng. 2003;83:321–34.

11. Senger RS, Karim MN. Effect of shear stress on intrinsic CHO culture state and glycosylation of recombinant tissue-type plasminogen activator protein. Biotechnol Progr. 2003;19:1199–209.

12. Wong DCF, Wong KTK, Goh LT, Heng CK, Yap MGS. Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. Biotechnol Bioeng. 2005;89:164–77.

13. Yang M, Butler M. Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms. Biotechnol Progr. 2002;18:129–38.

blog comments powered by Disqus



Novartis Reports Positive Results for Secukinumab in Ankylosing Spondylitis Trials
October 23, 2014
Pall ForteBio Releases Bioprocessing Contamination Detection Kit
October 22, 2014
Roche to Expand and Improve its Basel Site
October 22, 2014
FDA Panel Unanimously Backs Secukinumab for the Treatment of Psoriasis
October 22, 2014
EMA Works to Speed Up Ebola Treatment
October 20, 2014
Author Guidelines
Source: BioPharm International Supplements,
Click here