PEG Precipitation: A Powerful Tool for Monoclonal Antibody Purification - This alternative purification method to chromatography is readily scalable and fits a fully disposable downstream process. - B

ADVERTISEMENT

PEG Precipitation: A Powerful Tool for Monoclonal Antibody Purification
This alternative purification method to chromatography is readily scalable and fits a fully disposable downstream process.


BioPharm International Supplements


References

1. Cohn EJ, Strong LE, Hughes WL, Mulford DJ, Ashworth JN, Melin M, et al. Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids. J Amer Chem Soc. 1946;68(3):459–75.

2. Van der Wielen L, Hofland G, Ottens M, Gulobovic M, Witkam G-J. Manufacturing protein-based structures using a volatile acid. In: 224th National Meeting of the American Chemical Society. Boston, MA; 2002.

3. Habeeb A, Francis R. Preparation of human immunoglobulin by ammonium sulfate precipitation. Vox Sanguinis. 1976;31:423–34.

4. Wang J, Diehl T, Aguiar D, Dai X-P, Arunakumari A. Precipitation of process-derived impurities in non-Protein A purification schemes for antibodies. BioPharm Int. 2009 Oct suppl, Downstream Processing 2010: Embracing Innovation;4–10.

5. McDonald P, Victa C, Carter-Franklin JN, Fahrner R. Selective antibody precipitation using polyelectrolytes: A novel approach to the purification of monoclonal antibodies. Biotechnol Bioeng. 2009;102(4):1141–51.

6. Moya W, Jaber J, Moya W, inventors; Millipore Corp., assignee. Purification of Proteins. United States patent US WO/2008/079280. 2006.

7. Polson A, inventor; South African Inventions Development Corporation, assignee. Fractionation of mixtures of proteinaceous substances using polyethylene glycol. United States patent US 3415804. 1968.

8. Giese G. Polyethylene glycol precipitation of monoclonal antibodies and the impact on column chromatography. In: BioProcess Int. Raleigh, NC; 2009.

9. Thrash S, Otto J, Deits T. Effect of divalent ions on protein precipitation with polyethylene glycol: mechanism of action and applications. Protein expression and purification. 1991;2(1):83–9.

10. Ramanan S, Stenson R, Ramanan S, inventors; Amgen, assignee. Method of isolating antibodies by precipitation. United States patent US 2008/0214795 A1. 2008 2/13/08.

11. Venkiteshwaran A, Heider P, Teysseyre L, Belfort G. Selective precipitation-assisted recovery of immunoglobulins from bovine serum using controlled-fouling crossflow membrane microfiltration. Biotechnol Bioeng. 2008;101(5):957–66.

12. Fallaux FJ, Hoeben RC, Eb AJvd, Bout A, Valerio D, Fallaux FJ, Hoeben RC, inventors; IntroGene B.V, assignee. Packaging systems for human recombinant adenovirus to be used in gene therapy. United States patent US 5994128. 1997.

13. Golden K, Bragg C, Chon J. The XD process: development of a high titer process for PER.C6 cells. In: 21st Meeting of the European Society of Animal Cell Technology. Dublin, Ireland; 2009.

14. Chon J. Advances in platform fed-catch and XD production processes using the PER.C6 human cell line. Wilbio Waterside Conference; 2009 Apr 20–22; South San Francisco, CA.

15. Schirmer EB, Kuczewski M, Golden K, Lain B, Bragg C, Chon J, et al. Primary clarification of extreme-density cell culture harvests by enhanced cell settling. Bioprocess Int. 2010;8(1):32–9.

16. Lain B, Cacciuttolo MA, Zarbis-Papastoitsis G. Development of a High-Capacity MAb Capture Step Based on Cation-Exchange Chromatography. Bioprocess Int. 2009;7(5):26–34.

17. Yigzaw Y, Piper R, Tran M, Shukla AA. Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purification. Biotechnol Prog. 2006;22:288–96.

18. Milby KH, Ho SV, Henis JMS. Ion-exchange chromatography of proteins: the effect of neutral polymers in the mobile phase. J Chromatogr. 1989;482:133–44.

19. Gagnon P, Godfrey B, Ladd D. Method for obtaining unique selectivities in ion-exchange chromatography by addition of organic polymers to the mobile phase. J Chromatogr A. 1996;743:51–5.

20. Paithankar KR, Prasad KS. Precipitation of DNA by polyethylene glycol and ethanol. Nucleic Acids Research.1991;19(6):1346.


blog comments powered by Disqus

ADVERTISEMENT

ADVERTISEMENT

Pfizer to Acquire Vaccines from Baxter
July 30, 2014
GSK Submits EU Regulatory Filing for Malaria Vaccine Candidate
July 29, 2014
Bristol-Myers Squibb and Ono Pharmaceutical Collaborate on Immunotherapies
July 28, 2014
FDA Accepts First Biosimilar Filing
July 24, 2014
Compounding Pharmacy Issues Recall, But Challenges FDA Decision
July 22, 2014
Author Guidelines
Source: BioPharm International Supplements,
Click here