hemoglobin A complex protein (a 4-mer) in red blood cells that binds and releases oxygen, carrying it from the lungs to all other tissues.
HEPA filtration High-efficiency particulate air filter used to remove contaminants or to prevent particles from entering a clean room.
heterogeneity A term used to describe a biological component (i.e. protein) consisting of multiple structural variations.
Human growth hormone; an early biopharmaceutical. Formerly derived from cadaveric pituitary glands, this protein is now produced by recombinant
Hydrophobic interaction chroma-tography; a type of liquid chromatography that makes use of the relative solubility of proteins and matrix materials. Hydrophobic interactions
are strongest at high ionic strengths, so salt is usually included to increase those levels.
high-throughput screening Robotic methods used to sort through thousands of chemical compounds by running assays on many at a time.
Histidine; one of more than 20 naturally occurring amino acids.
histochemistry A science that combines the techniques of biochemistry and histology to deal with the chemical changes and chemical constitution
hit In drug discovery, a positive result in a high-throughput assay.
hormone A protein released by endocrine glands or sex organs to travel in the blood and act on tissues at another location in the
host A cell or organism used for growth of a virus, plasmid, or for the production of cloned substances.
host cell protein(s) (HCP) Proteins from the host cell system used to manufacture a biopharmaceutical drug. Host cells contain hundreds to thousands
of host cell proteins and other biomolecules that could contaminate the final product.
High-performance liquid chromatography or high-pressure liquid chromatography; a form of liquid chromatography in which a sample is forced at high pressure through a tube (column) that is packed tightly
with chromatographic media.
human genome The complete set of human genetic instructions.
High Definition Mass Spectrometry, is possible with the Waters SYNAPTTM G2 HDMS System. It combines high efficiency ion mobility-based
measurements and separations with quadrupole time-of flight (TOF) mass spectrometry, enabling researchers to differentiate
samples by size, shape, charge, and mass.
hybridization The process of joining complementary strands of DNA to make an RNA-DNA hybrid; the partial pairing of DNA single strands from
genetically different sources.
hybridoma An immortalized cell line (usually derived by fusing B-lymphocyte cells with myeloma tumor cells) that secretes desirable
hydrolysis Literally "cleaved by water," a reaction in which the chemical bond attaching an atom or group of atoms to the rest of a molecule
is severed, followed by attachment of hydrogen at the same point. Most often in biopharmaceuticals, the breakage of peptide
bonds by addition of a water molecule.
hydrophilic Having an affinity for water; attracting, dissolving in, or absorbing water; readily absorbing moisture; having strongly polar
molecular groups that readily interact with water.
hydrophobic Insoluble in water; the extent of insolubility; not readily absorbing water; resisting or repelling water, wetting, or hydra-tion;
or being adversely affected by water.
hydrophobicity The lack of an affinity for water.
hygroscopic Ready to take up and retain moisture.