CONCLUSION AND OUTLOOK
The principle of the MCSGP process and its characteristics with respect to existing technologies, such as SMB, has been explained
and promising areas of application have been discussed. For polypeptide purification, a batch chromatography step has been
compared to the experimental results of the MCSGP unit. It has been shown that strong performance improvement in yield and
productivity are possible in the MCSGP setup. The best MCSGP operating point showed a 25-fold improvement in productivity
and yields that were 5–7% higher than the batch purification.
Because of the significant potential of the MCSGP process to reduce the cost of chromatographic steps for challenging biomolecule
purifications, further applications are foreseeable.
Lars Aumann, PhD, is chief technology officer, Guido Stroehlein, PhD, is chief executive officer, and Thomas Mueller-Spaeth, PhD, is chief scientific officer, all at ChromaCon AG, Zürich, Switzerland, +41 44 633 7748, lars.aumann@chromacon.ch
Massimo Morbidelli, PhD, is professor for chemical and bioengineering at ETH Zürich, Zürich, Switzerland. Berthold Schenkel, PhD, is head of technology group 1, CHBS, at Novartis Pharma AG, Basel, Switzerland.
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