1. Enders JF, Weller TH, Robbins FC. Cultivation of Lansing strain of poliomyelitis virus in culture of various human embryonic
tissues. Science. 1949;109:85–87.
2. Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122:501–504.
3. Goswami J, et al. Apoptosis in batch cultures of Chinese hamster ovary cells. Biotech Bioeng. 1999;62:632–640.
4. Merten OW. Development of serum-free media for cell growth and production of viruses/viral vaccines—safety issues of animal
products used in serum-free media. Dev Biol. 2002;111:233–257.
5. Castle P, Robertson, JS. Animal sera, animal sera derivatives and substitutes used in the manufacture of pharmaceuticals:
viral safety and regulatory aspects. Dev Biol Stand. 1999;99:191–196.
6. Wessman SJ, Levings RL. Benefits and risks due to animal serum used in cell culture production. Dev Biol Stand. 1999;99:3–8.
7. Hewlett G. Strategies for optimizing serum-free media. Cytotechnol. 1991;5:3–14.
8. Ham RG. Clonal growth of mammalian cells I chemically defined, synthetic medium. Proc Natl Acad Sci. 1965;53:288–295.
9. Chang TH, et al. Production of monoclonal antibodies in serum-free medium. J Immunol Methods. 1980;39:369–375.
10. Gu X, Xie L, Harmon BJ, Wang DIC. Influence of Primatone RL supplementation on sialylation of recombinant human interferon-Y
produced by Chinese hamster ovary cell culture using serum-free media. Biotechnol Bioeng. 1997;56(4):353–360.
11. Sung YH, Lim SW, Chung JY, Lee GM, Yeast hydrolysate as a low-cost alternative to serum-free medium for the production
of human thrombopoietin in suspension cultures. Appl Micrbiol Biotechnol. 2004;63:527–536.
12. Franek F, Hohenwarter O, Katinger H. Plant protein hydrolysates: preparation of defined peptide fractions promoting growth
and production in animal cell culture. Biotechnol Prog. 2000;16:88–692.
13. Chun BH, Kim JH, Lee HJ, Chung Namhyun. Usability of size-excluded fractions of soy protein hydrolysates for growth and
viability of Chinese hamster ovary cells in protein-free suspension culture. Bioresource Technol. 2007;98:1000–1005.
14. Chabanon G, et al. Influence of the rapeseed protein hydrolysis process on CHO cell growth. Bioresource Technol. 2008
15. Babcock J, et al. Enhancing performance in cell culture. Gen Eng Biotechnol News. 2007 Nov 15;47–48.
16. Chuang VT, Kragh-Hansen U, Otagiri M. Pharmaceutical strategies utilizing recombinant human serum albumin. Pharm Res.
17. Bosse D, Praus M, Kiessling P, Nyman L, Andersen C, Waters J, Schindel F. Phase I comparability of recombinant human
albumin and human serum albumin. J Clin Pharmacol. 2005;45:57–67.
18. Griffiths JB, The effect of insulin on the growth and metabolism of human diploid cell, WI-38. J Cell Sci. 1970;7:575–585.
19. Drapeau D. Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process.
20. Goswami J. Apoptosis in batch cultures of Chinese hamster ovary cells. Biotech Bioeng. 1999;62:632–640.
21. Van Wyk JJ, Graves DC, Casella SJ, and Jacobs S. Evidence from monoclonal antibody studies that insulin stimulates DNA
synthesis through the type I somatomedin receptor. J Clin Endocrinol Metab. 1985 Oct;61(4):639–43.
22. Kim DY, Lee JC, Chang HN, Oh DJ. Effects of supplementation of various medium components on Chinese hamster ovary cell
cultures producing recombinant antibody. Cytotechnol. 2005;47:37–49.
23. Yandell C, Lawson J, Butler I, Wade B, Sheehan A, Grosvenor S, Goddard C, Simula T. An analogue of IGF-I. A potent substitute
for insulin in serum-free manufacture of biologics by CHO cells. BioProcess Int. 2004 Mar;2–7.
24. Morris AE, Schmid J. Effects of insulin and LONG R3IGF-I on serum-free Chinese hamster ovary cell cultures expressing
two recombinant proteins. Biotechnol Prog. 2000;16:693–697.
25. Richardson DR, Ponka P. The molecular mechanism of the metabolism of iron in normal and neoplastic cells. Biochem Biophys
26. Conrad ME, Umbreit JN, Morre EG, Iron absorption and transport. Am J Med Sci. 1994;318:213–229.
27. Keenan J, Pearson D, Lorraine O, Gammell P, Clynes M. Evaluation of recombinant human transferrin (DeltaFerrin) as an
iron chelator in serum-free media for mammalian cell culture. Cytotechnol. 2006;51:29–37.
28. Grosvenor S, Chirkova L, Standfield S, Voorhamme D. Enhance CHO cell performance with a combination of CellPrime recombinant
Transferrin and LONG R3 IGF-I. BioProcessing J. 2007(Winter);6(4):45–51.
29. Sunstrom NA, Gay RD, Wong DC, Kitchen NA, Deboer L, Gray PP. Insulin-like growth factor-I and transferring mediate growth
and survival of Chinese hamster ovary cells. Biotech Prog. 2000;16:698–702.