Harvest and Recovery of Monoclonal Antibodies from Large-Scale Mammalian Cell Culture - Comparing primary harvest techniques adopted in commercial-scale operations for monoclonal antibody products. -

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Harvest and Recovery of Monoclonal Antibodies from Large-Scale Mammalian Cell Culture
Comparing primary harvest techniques adopted in commercial-scale operations for monoclonal antibody products.


BioPharm International
Volume 21, Issue 5

19. Knight, R, Ostreicher, E. Charge-modified filter media. In: Meltzer, TH, Jornitz, MW, editors. Filtration in the Biopharmaceutical Industry. New York: Marcel Dekker; 1998. p. 95–125.

20. Gerba C, Hou K. Endotoxin removal by charge-modified filters. Appl Environ Microbiol. 1985; 50:1375–1377.

21. Hou K, Gerba C, Goyal S, Zerda K. Capture of latex beads, bacteria, endotoxin and viruses by charge-modified filters. Appl Environ Microbiol. 1980;40:892 –896.

22. Dorsey N, Eschrich J, Cyr G. The role of charge in the retention of DNA by charged cellulose based depth filters. Biopharm. 1997(1);46–49.

23. Tipton B, Boose JA, Larsen W, Beck J, O'Brien T. Retrovirus and parvovirus clearance from an affinity column product using adsorptive depth filtration. Biopharm. 2002(9):43–50.

24. Yigzaw Y, Tranh M, Piper R, Shukla A. Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purification. Biotechnol Prog. 2006;22:288–296.

25. Yavorsky D, Blanck R, Lambalot C, Brunkow R. The clarification of bioreactor cell cultures for biopharmaceuticals. Pharm Technol. 2003(3):62–74.

26. Riske F, Schroeder J, Belliveau J, Kang X, Kutzko J, Menon M. The use of chitosan as a flocculant in mammalian cell culture dramatically improves clarification throughput without adversely affecting monoclonal antibody recovery. J Biotechnol. 2007;128:813–823.

27. Coffman J. Flocculation of antibody-producing mammalian cells with precipitating solutions of soluble cations and anions. International Conference on Recovery of Biological Products XII. Abstact # M3; 2006 Apr 2 –7; Litchfield, Arizona.

28. Thommes J, Bader A, Halfar M, Karau A, Kula M. Isolation of mAbs from cell containing hybridoma broth using protein A coated adsorbent in expanded beds. J Chromatogr. 1996;153:111–122.

29. Hjorth R. Expanded-bed adsorption in industrial bioprocessing: recent developments. Tibtech. 1997;15:230–235.

30. Anspach F, Curbelo D, Hartmann R, Garke G, Deckwer WD. Expanded-bed chromatography in primary protein purification. J Chromatogr A. 1999;865:129–144.

31. Sonnenfeld A, Thommes J. Expanded bed adsorption for capture from crude solution. In: Shukla AA, Etzel MR, Gadam S, editors. Process scale bioseparations for the biopharmaceutical industry. Boca Raton (FL): CRC Press; 2007. p. 59–81.

32. Wang A, Lewos R, Rathore A. Comparisons of different options for harvest of a therapeutic protein product from high cell density yeast fermentation broth. Biotechnol Bioeng. 2006;94:91–104.


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