Process-Scale Precipitation of Impurities in Mammalian Cell Culture Broth - If certain engineering challenges can be addressed, precipitation may prove to be a valuable tool for antibody

ADVERTISEMENT

Process-Scale Precipitation of Impurities in Mammalian Cell Culture Broth
If certain engineering challenges can be addressed, precipitation may prove to be a valuable tool for antibody purification


BioPharm International Supplements


References

1. Przybycien T, Narahari S, Steele L. Alternative bioseparation operations: life beyond packed-bed chromatography. Current Opin Biotechnol. 2004;15:469–478.

2. Akcasu A, Akcasu M, Tumay S. A new method for the differential precipitation of nucleic acids from aqueous solutions. Nature. 1960;4734:23–324.

3. Matsuzawa Y, Hiraishi A. Study on DNA precipitation with a cationic polymer PAC (poly aluminum chloride). Nucleic Acids Res Supplement. 2003;3:163–164.

4. Christensen P, Danielczyk, A, Stahn R, Goletz S. Simple separation of DNA in antibody purification. Protein Expr Purif. 2004; 37:468–471.

5. Kejnovsky E, Kypr J. DNA extraction by zinc. Nucleic Acids Res. 1997;25(9):1870–1871.

6. Raspaud E, de la Cruz O, Sikorav JL, Livolant F. Precipitation of DNA by polyamines: a polyelectrolyte behavior. Biophys J. 1998;74:381–393.

7. Choe W, Middelberg A. Selective precipitation of DNA by spermine during the chemical extraction of insoluble cytoplasmic protein. Biotechnol Prog. 2001;17:1107–1113.

8. Clark K, Glatz C. Polymer dosage considerations in polyelectrolyte precipitation of protein. Biotechnol Prog. 1987;3(4):241–247.

9. Cordes R, Sims W, Glatz C. Precipitation of nucleic acids with poly(ethyleneimine). Biotechnol Prog. 1990;6:283–285.

10. Dissing U, Mattiasson B. Integrated removal of nucleic acids and recovery of LDH from homogenate of beef heart by affinity precipitation. Bioseparation. 1999;7:221–229.

11. Del Sal G, Manfioletti G, Schneider C. The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages, or plasmids suitable for sequencing. BioTechniques. 1989;7(5):514–519.

12. Goerke A, To B, Lee A, Sagar S, Konz J. Develop of a novel adenovirus purification process utilizing selective precipitation of cellular DNA. Biotechnol Bioeng. 2005;91(1):12–21.

13. Persson J, Lester P. Purification of antibody and antibody-fragment from E. coli homogenate using 6,9-diamino-2-ethoxyacridine lactate as precipitation agent. Biotechnol Bioeng. 2004;87(3):424–434.

14. Horejsi J, Smetana R. The isolation of á-globulin from blood serum by Rivanol. Acta Medica Scand. 1956;155:65–70.

15. Chanutin A, Curnish R. The precipitation of plasma proteins by short-chain fatty acids. Arch Biochem Biophys. 1960;89:218–220.

16. Habeeb A, Francis R. Preparation of human immunoglobulin by caprylic acid precipitation. Prep Biochem. 1984;14(1):1–17.

17. McKinney M, Parkinson A. A simple, nonchromatographic procedure to purify immunoglobulins from serum and ascites fluid. J Immunol Methods. 1987;96:271–278.

18. Reik L, Maines S, Ryan D, Levin W, Bandiera S, Thomas P. A simple, nonchromatographic purification procedure for monoclonal antibodies. J Immunol Methods. 1987;100:123–130.

19. Temponi M, Kageshita T, Perosa F, Ono R, Okada H, Ferrone S. Purification of murine IgG monoclonal antibodies by precipitation with caprylic acid: comparison with other methods of purification. Hybridoma. 1989;8(1):85–95.

20. Parkkinen J, Rahola A, von Bonsdorff L, Tölö H, Törmä E. A modified caprylic acid method for manufacturing immunoglobulin G from human plasma with high yield and efficient virus clearance. Vox Sang. 2006;90:97–104.

21. Lundblad J, Seng R. Inactivation of Lipid-Enveloped Viruses in Proteins by Caprylate. Vox Sang. 1991;60:75–81.

22. Habeeb A, Francis R. Preparation of human immunoglobulin by ammonium sulfate precipitation. Vox Sang. 1976;31:423–434.

23. Kent U. Purification of antibodies using ammonium sulfate fractionation or gel fractionation. Methods in Molecular Biology, 115, Humana Press, Inc., Totowa, New Jersey. 1999;pp. 11–18.

24. Page M, Thorpe R. Purification of IgG by precipitation with sodium sulfate or ammonium sulfate. The Protein Protocols Handbook, 2nd Edition, Humana Press, Inc., Totowa, New Jersey. 2002;pp. 983–984.

25. Shenoy V, Kulkarni M. Centrifuges in the process industry. Chem Ind Digest. 2006;19(2):63–68.

26. Kempken R, Preissmann A, Berthold, W. Assessment of a disk stack centrifuge for use in mammalian cell separation. Biotechnol Bioeng. 1995;46:132–138.

27. Shpritzer R, Acharya H, Petrone J, Vicik S. Evaluation of a continuous disc stack centrifuge for the clarification of mammalian cell cultures. The 225th ACS National Meeting. New Orleans, LA. 2003;Mar 23–27.

28. Glynn J, Zaman F, Chen B, Wealand J, Mensah P. Development of a MAb harvest protocol. Biochemical Engineering XV: Engineering Biology from Biomolecules to Complex Systems. Quebec City, Canada. 2007;Jul 15–19

29. Raghunath B. Cacace B, Yavorsky D. Strategies for optimizing upstream clarification of cell culture fluids. The 234th ACS National Meeting, Boston, MA. 2007;Aug 19–23.

30. Maybury J, Mannweiler K, Titchener-Hooker N, Hoare M, Dunnill P. The performance of a scaled down industrial disc stack centrifuge with a reduced feed material requirement. Bioprocess Eng. 1998;18:191–199.

31. Hutchinson N, Bingham N, Murrell N, Farid S, Hoare M. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol Bioeng. 2006;95(3):483–491.

32. Russell E, Wang A, Rathore A. Harvest of a therapeutic protein product from high cell density fermentation broths: principles and case study. Biotechnol and Bioprocess. 2007;31:1–58.

33. Duffy J, Mcnerny T, Wypych J. Centrifugation strategies for recovery of a mammalian expressed MAb. The 227th ACS National Meeting, Anaheim, CA. 2004;Mar 28–April1.

34. Bates R, Ma J, Mason C. Case study: optimization of harvest stream clarification steps of a commercially-relevant fusion protein mammalian cell culture using charged depth filters. The 234th ACS National Meeting, Boston, MA. 2007;Aug 19–23.

35. Prashad M, Tarrach K. Depth filtration: Cell clarification of bioreactor offloads. Filtration + Separation. 2006;43(7):28–30.

36. Charlton H, Relton J, Slater N. Characterization of a generic MAb harvesting system for adsorption of DNA by depth filters and various membranes. Bioseparation. 1999;8: 281–291.

37. Yigzaw Y, Piper R, Tran M, Shukla A. Exploitation of the adsorptive properties of depth filters for host cell protein removal during MAb purification. Biotechnol Progr. 2006;22:288–296.

38. Arnold T. Fluid purification using charge-modified depth filtration media. Bioprocess Int. 2005;3(10):44–49.


blog comments powered by Disqus

ADVERTISEMENT

ADVERTISEMENT

FDA Approves Pfizer's Trumenba for the Prevention of Meningitis B
October 30, 2014
EMA: Extrapolation Across Indications for Biosimilars a Possibility
October 30, 2014
Bristol-Myers Squibb Announces Agreement to Acquire HER2-Targeted Cancer Treatment
October 29, 2014
Contract Research and Manufacturing Organization Paragon Bioservices Raises $13 Million
October 28, 2014
Yale and Gilead Extend Sequencing Initiative
October 28, 2014
Author Guidelines
Source: BioPharm International Supplements,
Click here