Process-Scale Precipitation of Impurities in Mammalian Cell Culture Broth - If certain engineering challenges can be addressed, precipitation may prove to be a valuable tool for antibody


Process-Scale Precipitation of Impurities in Mammalian Cell Culture Broth
If certain engineering challenges can be addressed, precipitation may prove to be a valuable tool for antibody purification

BioPharm International Supplements


1. Przybycien T, Narahari S, Steele L. Alternative bioseparation operations: life beyond packed-bed chromatography. Current Opin Biotechnol. 2004;15:469–478.

2. Akcasu A, Akcasu M, Tumay S. A new method for the differential precipitation of nucleic acids from aqueous solutions. Nature. 1960;4734:23–324.

3. Matsuzawa Y, Hiraishi A. Study on DNA precipitation with a cationic polymer PAC (poly aluminum chloride). Nucleic Acids Res Supplement. 2003;3:163–164.

4. Christensen P, Danielczyk, A, Stahn R, Goletz S. Simple separation of DNA in antibody purification. Protein Expr Purif. 2004; 37:468–471.

5. Kejnovsky E, Kypr J. DNA extraction by zinc. Nucleic Acids Res. 1997;25(9):1870–1871.

6. Raspaud E, de la Cruz O, Sikorav JL, Livolant F. Precipitation of DNA by polyamines: a polyelectrolyte behavior. Biophys J. 1998;74:381–393.

7. Choe W, Middelberg A. Selective precipitation of DNA by spermine during the chemical extraction of insoluble cytoplasmic protein. Biotechnol Prog. 2001;17:1107–1113.

8. Clark K, Glatz C. Polymer dosage considerations in polyelectrolyte precipitation of protein. Biotechnol Prog. 1987;3(4):241–247.

9. Cordes R, Sims W, Glatz C. Precipitation of nucleic acids with poly(ethyleneimine). Biotechnol Prog. 1990;6:283–285.

10. Dissing U, Mattiasson B. Integrated removal of nucleic acids and recovery of LDH from homogenate of beef heart by affinity precipitation. Bioseparation. 1999;7:221–229.

11. Del Sal G, Manfioletti G, Schneider C. The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages, or plasmids suitable for sequencing. BioTechniques. 1989;7(5):514–519.

12. Goerke A, To B, Lee A, Sagar S, Konz J. Develop of a novel adenovirus purification process utilizing selective precipitation of cellular DNA. Biotechnol Bioeng. 2005;91(1):12–21.

13. Persson J, Lester P. Purification of antibody and antibody-fragment from E. coli homogenate using 6,9-diamino-2-ethoxyacridine lactate as precipitation agent. Biotechnol Bioeng. 2004;87(3):424–434.

14. Horejsi J, Smetana R. The isolation of á-globulin from blood serum by Rivanol. Acta Medica Scand. 1956;155:65–70.

15. Chanutin A, Curnish R. The precipitation of plasma proteins by short-chain fatty acids. Arch Biochem Biophys. 1960;89:218–220.

16. Habeeb A, Francis R. Preparation of human immunoglobulin by caprylic acid precipitation. Prep Biochem. 1984;14(1):1–17.

17. McKinney M, Parkinson A. A simple, nonchromatographic procedure to purify immunoglobulins from serum and ascites fluid. J Immunol Methods. 1987;96:271–278.

18. Reik L, Maines S, Ryan D, Levin W, Bandiera S, Thomas P. A simple, nonchromatographic purification procedure for monoclonal antibodies. J Immunol Methods. 1987;100:123–130.

19. Temponi M, Kageshita T, Perosa F, Ono R, Okada H, Ferrone S. Purification of murine IgG monoclonal antibodies by precipitation with caprylic acid: comparison with other methods of purification. Hybridoma. 1989;8(1):85–95.

20. Parkkinen J, Rahola A, von Bonsdorff L, Tölö H, Törmä E. A modified caprylic acid method for manufacturing immunoglobulin G from human plasma with high yield and efficient virus clearance. Vox Sang. 2006;90:97–104.

21. Lundblad J, Seng R. Inactivation of Lipid-Enveloped Viruses in Proteins by Caprylate. Vox Sang. 1991;60:75–81.

22. Habeeb A, Francis R. Preparation of human immunoglobulin by ammonium sulfate precipitation. Vox Sang. 1976;31:423–434.

23. Kent U. Purification of antibodies using ammonium sulfate fractionation or gel fractionation. Methods in Molecular Biology, 115, Humana Press, Inc., Totowa, New Jersey. 1999;pp. 11–18.

24. Page M, Thorpe R. Purification of IgG by precipitation with sodium sulfate or ammonium sulfate. The Protein Protocols Handbook, 2nd Edition, Humana Press, Inc., Totowa, New Jersey. 2002;pp. 983–984.

25. Shenoy V, Kulkarni M. Centrifuges in the process industry. Chem Ind Digest. 2006;19(2):63–68.

26. Kempken R, Preissmann A, Berthold, W. Assessment of a disk stack centrifuge for use in mammalian cell separation. Biotechnol Bioeng. 1995;46:132–138.

27. Shpritzer R, Acharya H, Petrone J, Vicik S. Evaluation of a continuous disc stack centrifuge for the clarification of mammalian cell cultures. The 225th ACS National Meeting. New Orleans, LA. 2003;Mar 23–27.

28. Glynn J, Zaman F, Chen B, Wealand J, Mensah P. Development of a MAb harvest protocol. Biochemical Engineering XV: Engineering Biology from Biomolecules to Complex Systems. Quebec City, Canada. 2007;Jul 15–19

29. Raghunath B. Cacace B, Yavorsky D. Strategies for optimizing upstream clarification of cell culture fluids. The 234th ACS National Meeting, Boston, MA. 2007;Aug 19–23.

30. Maybury J, Mannweiler K, Titchener-Hooker N, Hoare M, Dunnill P. The performance of a scaled down industrial disc stack centrifuge with a reduced feed material requirement. Bioprocess Eng. 1998;18:191–199.

31. Hutchinson N, Bingham N, Murrell N, Farid S, Hoare M. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol Bioeng. 2006;95(3):483–491.

32. Russell E, Wang A, Rathore A. Harvest of a therapeutic protein product from high cell density fermentation broths: principles and case study. Biotechnol and Bioprocess. 2007;31:1–58.

33. Duffy J, Mcnerny T, Wypych J. Centrifugation strategies for recovery of a mammalian expressed MAb. The 227th ACS National Meeting, Anaheim, CA. 2004;Mar 28–April1.

34. Bates R, Ma J, Mason C. Case study: optimization of harvest stream clarification steps of a commercially-relevant fusion protein mammalian cell culture using charged depth filters. The 234th ACS National Meeting, Boston, MA. 2007;Aug 19–23.

35. Prashad M, Tarrach K. Depth filtration: Cell clarification of bioreactor offloads. Filtration + Separation. 2006;43(7):28–30.

36. Charlton H, Relton J, Slater N. Characterization of a generic MAb harvesting system for adsorption of DNA by depth filters and various membranes. Bioseparation. 1999;8: 281–291.

37. Yigzaw Y, Piper R, Tran M, Shukla A. Exploitation of the adsorptive properties of depth filters for host cell protein removal during MAb purification. Biotechnol Progr. 2006;22:288–296.

38. Arnold T. Fluid purification using charge-modified depth filtration media. Bioprocess Int. 2005;3(10):44–49.

blog comments powered by Disqus



GPhA Issues Statement on Generic Drug Costs
November 20, 2014
Amgen Opens Single-Use Manufacturing Plant in Singapore
November 20, 2014
Manufacturing Issues Crucial to Combating Ebola
November 20, 2014
FDA Requests Comments on Generic Drug Submission Criteria
November 20, 2014
USP Joins Chinese Pharmacopoeia Commission for Annual Science Meeting
November 20, 2014
Author Guidelines
Source: BioPharm International Supplements,
Click here