The Evolution of Protein Expression and Cell Culture - - BioPharm International


The Evolution of Protein Expression and Cell Culture

BioPharm International
Volume 20, Issue 10

20. Bode J, Schlake T, Iber M, Schübeler D, Seibler J, Snezhkov E, Nikolaev L. The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes. J Biol Chem. 2000 Sep–Oct;381(9–10):801–13.

21. Figueroa B, Jr., Ailor E, Osborne D, Hardwick JM, Reff M, Betenbaugh MJ: Enhanced cell culture performance using inducible anti-apoptotic genes E1B-19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells. Biotechnol Bioeng. 2007;97(4):877–892.

22. Lee KH, Sburlati A, Renner WA, Bailey JE. Deregulated expression of cloned transcription factor E2F-1 in Chinese hamster ovary cells shifts protein patterns and activates growth in protein-free medium. Biotechnol Bioeng. 1996;50(3):273–279.

23. Ifandi V, Al-Rubeai M. Regulation of cell proliferation and apoptosis in CHO-K1 cells by the coexpression of c-Myc and Bcl-2. Biotechnol Prog. 2005 May–Jun;21(3):671–7.

24. Jaluria P, Betenbaugh M, Konstantopoulos K, Frank B, Shiloach J. Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells. Metab Eng. 2007 May;9(3):241–51.

25. Borth N, Mattanovich D, Kunert R, Katinger H. Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. Biotechnol Prog. 2005 Jan–Feb;21(1):106–11.

26. Umaña P, Jean-Mairet J, Moudry R, Amstutz H, Bailey JE. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity. Nat Biotechnol. 1999 Feb;17(2):176-80.

27. DeMaria CT, Cairns V, Schwarz C, Zhang J, Guerin M, Zuena E, et al. Accelerated clone selection for recombinant CHO cells using a FACS-based high-throughput screen. Biotechnol Prog. 2007 Mar–Apr;23(2):465–72.

28. Seth G, Philp RJ, Lau A, Jiun KY, Yap M, Hu WS. Molecular portrait of high productivity in recombinant NS0 cells. Biotechnol Bioeng. 2007 Jul 1;97(4):933–51.

29. Barnes LM, Bentley CM, Moy N, Dickson AJ. Molecular analysis of successful cell line selection in transfected GS-NS0 myeloma cells. Biotechnol Bioeng. 2007 Feb 1;96(2):337–48.

30. Zhang, Jinyou, Robinson, David. Development of animal-free, protein-free and chemically-defined media for NS0 cell culture. Cytotechnol. 2005 Jun;48(1–3):59–74.

31. Walowitz JL, Fike RM, Jayme DW. Efficient lipid delivery to hybridoma culture by use of cyclodextrin in a novel granulated dry-form medium technology. Biotechnol Prog. 2003 Jan–Feb;19(1):64–8.

32. Burky JE, Wesson MC, Young A, Farnsworth S, Dionne B, Zhu Y, et al. Protein-free fed-batch culture of non-GS NS0 cell lines for production of recombinant antibodies. Biotechnol Bioeng. 2007 Feb 1;96(2):281–93.

33. Seth G, Ozturk M, Hu WS. Reverting cholesterol auxotrophy of NS0 cells by altering epigenetic gene silencing. Biotechnol Bioeng. 2006 Mar 5;93(4):820–7.

34. Ge X, Hanson M, Shen H, Kostov Y, Brorson KA, Frey DD, Moreira A., Rao G. Validation of an optical sensor-based high-throughput bioreactor system for mammalian cell culture. J Biotechnol. 2006 Apr 10;122(3):293–306.

35. Allison DW, Aboytes KA, Fong DK, Leugers SL, Johnson TK, Loke HN, et al. Development and optimization of cell culture media: Genomic and proteomic approaches. BioProcess Int. 2005 Jan;3(1):38–45.

blog comments powered by Disqus



Bristol-Myers Squibb and Five Prime Therapeutics Collaborate on Development of Immunomodulator
November 26, 2014
Merck Enters into Licensing Agreement with NewLink for Investigational Ebola Vaccine
November 25, 2014
FDA Extends Review of Novartis' Investigational Compound for Multiple Myeloma
November 25, 2014
AstraZeneca Expands Biologics Manufacturing in Maryland
November 25, 2014
GSK Leads Big Pharma in Making Its Medicines Accessible
November 24, 2014
Author Guidelines
Source: BioPharm International,
Click here