ALTERNATIVES TO PROTEIN A: Improved Downstream Process Design for Human Monoclonal Antibody Production - A two-step process—a cation exchange capture column followed by anion exchange flow-throu

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ALTERNATIVES TO PROTEIN A: Improved Downstream Process Design for Human Monoclonal Antibody Production
A two-step process—a cation exchange capture column followed by anion exchange flow-through membrane chromatography—resulted in >80% process yield, higher batch capacity, and shorter process time.


BioPharm International


References

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2. Gottschalk U. The renaissance of protein purification. Supplement to BioPharm Int. 2006 June:8–9.

3. Sofer G, Chirica LC. Improving productivity in downstream processing. BioPharm Int. 2006 Nov 19; (11):48–55.

4. Zhou JX, Tressel T. Membrane chromatography as a robust purification system for large-scale antibody production. Supplement to BioProcess Int. 2005 Sept 3; (9):32–37.

5. Cacciuttolo MA, Arunakumari A. Scale-up considerations for biotechnology-derived products. In: Michael Levin, editor. Drugs and the pharmaceutical Sciences, Vol 157, Pharmaceutical process scale-up, 2nd ed. Chapter 5. Boca Raton, FL: CRC Press, 2006.

6. Arunakumari A. Integrating high titer cell culture processes with highly efficient purification processes for the manufacturing of human monoclonal antibodies. BioProcess International Conference. San Francisco CA; 2006 Nov 6–9.

7. Jagschies G, Grönberg A, Björkman T, Lacki K, Johansson HJ. Technical and economical evaluation of downstream processing options for monoclonal antibody (MAb) production. Supplement to BioPharm Int. 2006 June:10–19.


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