PEGylation of Proteins: A Structural Approach - Structural properties of PEGylated proteins could play an increasingly important role in developing optimal therapeutic protein drugs. - BioPharm


PEGylation of Proteins: A Structural Approach
Structural properties of PEGylated proteins could play an increasingly important role in developing optimal therapeutic protein drugs.

BioPharm International
Volume 19, Issue 4

Although PEGylation remains an excellent choice for protein stabilization and controlled dosage of protein drugs, however, it still poses a number of challenges. Manufacturing costs of goods, PEG polydispersity, drug clearance from the body, and loss of biological activity resulting from PEGylation are just a few of the areas in need of further investigation. Because of the improvement of PEG purification processes, the commercially available PEGs are nowadays less polydisperse, allowing for larger molecular weight polymers to be used. In addition, the use of branched PEGs and the development of robust site specific PEGylation have expanded and will continue to expand the polymer options available for protein PEGylation. Bigger and better PEGs would require better characterization of the overall structural properties of the PEG and the conjugated complex. A conformational approach to PEGylated species equilibrium offers insight into alternative options for optimization of the PEGylation reaction.

A. Sorina Morar, PhD, is a scientist, Process Development, Diosynth Biotechnology, 3000 Weston Parkway, Carey, NC 25713, 919.388.5649, fax: 919.678.0366,


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