Expression of Recombinant Proteins in Yeast - - BioPharm International


Expression of Recombinant Proteins in Yeast

10 Dumont J, Legrain M, Portetelle D, Brasseur R, Burny A, Hilger F. High yield synthesis of the bovine leukemia virus (BLV) p24 major internal protein in Saccharomyces cerevisiae. Gene 1989; 79(2):219-26.

11 Nishizawa M, Ozawa F, Hishinuma F. Construction of an expression and secretion vector for the yeast Saccharomyces cerevisiae. Agric. Biol. Chem. 1987; 51:515-521.

12 Chaudhuri B, Steube K, Stephan C. The pro-region of the yeast prepro-alpha-factor is essential for membrane translocation of human insulin-like growth factor 1 in vivo. European J. of Biochem. 1992; 206(3):793-800.

13 Pignatelli R, Vai M, Alberghina L, Popolo L. Expression and secretion of beta-galactosidase in Saccharomyces cerevisiae using the signal sequences of GgpI, the major yeast glycosylphosphati-dylinositol-containing protein. Biotechnol. Appl. Biochem. 1998; 27(Pt 2):81-8.

14 Higgins DR, Cregg JM. Introduction to Pichia pastoris. In: Higgins DR and Cregg JM eds. Pichia protocols, methods in molecular biology. Vol. 103. Totowa NJ: Humana Press, 1998.

15 Trinh LB, Phue JN, Shiloach J. Effect of methanol feeding strategies on recombinant mouse endostatin. Biotechnology and Bioengineering 2003; 82(4):438-444.

16 Vassileva A, Chugh DA, Swaminathan S, Khanna N. Expression of hepatitis B surface antigen in the methylotropic yeast Pichia pastoris using the GAP promoter. J. of Biotechnology 2001; 88:21-35.

17 Shen S, Sulter G, Jeffries TW, Cregg JM. A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia Pastoris. Gene 1998; 216(1):93-102.

18 Stratton J, Chiruvolu V, Meagher M. High cell-density fermentation. In: Higgins DR and Cregg JM eds. Pichia protocols. op cit.

19 Choi BK, Bobrowicz P, Davidson RC, Hamilton SR, Kung DH, Li H, et al. Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc. Natl. Acad. Sci. USA. 2003; 100(9):5022-5027.

20 Hamilton SR, Bobrowicz P, Bobrowicz B, Davidson RC, Li H, Mitchell T, et al. Production of complex human glycoproteins in yeast. Science 2003; 301(5637):1244-1246.

21 Ferminan E, Dominguez A. Heterologous protein secretion directed by a repressible acid phosphatase system of Kluyveromyces lactis: characterization of upstream region-activating sequences in the KIPHO5 gene. Appl. Environ. Microbiol. 1998; 64(7): 2403-2408.

blog comments powered by Disqus



AbbVie/Shire Deal Officially Off
October 20, 2014
Amgen Sues Sanofi and Regeneron over Patent for mAb Targeting PCSK9
October 20, 2014
EMA Works to Speed Up Ebola Treatment
October 20, 2014
Lilly to Close Manufacturing Facility in Puerto Rico
October 17, 2014
BioReliance Introduces New Predictive Assays
October 17, 2014
Author Guidelines
Source: BioPharm International,
Click here