Other Yeast Systems
A few other fungal systems have also been successfully used for recombinant protein production. Some of these systems are
based on non-fermentation yeast species like Pichia methanolica and Hansenula polymorpha. These are similar to Pichia pastoris and can be grown to very high cell densities and high-level protein expression has been achieved. There is also a wide range
of expression systems similar to the Pichia and Saccharomyces system available for recombinant protein expression. Table 3 shows some available expression technologies for these yeast
Table 3. Other yeast expression systems
This article is an excerpt from the chapter, "Advances in Improved Expression of Recombinant Proteins in Microbial Systems,"
in Eric S. Langer, Ed., Advances in Large-Scale Biopharmaceutical Manufacturing and Scale-Up Production (ASM Press, 2004).
Daniel Rudolph, Ph.D., is a process engineer in the process development group of Cambrex Bio Science Baltimore, Inc., 5901 E. Lombard Street,
Baltimore, MD 21224, 410.563.9200, Daniel.Rudolph@cambrex.com
. Co-authors Sriram Srinivasan, Ph.D., Don R. Durham, Ph.D., and Aaron Heifetz, Ph.D., previously served at Cambrex in the roles of process engineer, upstream process development group; vice president
of technology; and vice president, general manager, and site director, respectively.
1. Rudolph D, Srinivasan S, Durham DR, Heifetz A. Advances in improved ex-pression of recombinant proteins in microbial systems.
In: Langer ES, editor. Advances in large-scale biopharm-aceutical manufacturing and scale-up production. Volume 1: emerging tech-nologies and scientific advancements. Washington DC: ASM Press; 2004 October. p. 194-228.
2. Parekh RN, Shaw MR, Wittrup KD. An integrating vector for tunable, high copy, stable integration into the 1996dis-persed
Ty delta sites of Saccharomyces cerevisiae. Biotechnol. Prog. 1996; 12(1):16-21.
3 Nasmyth KA, Reed SL. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc. Natl. Acad. Sci. USA 1980; 77(4):2119-2123.
4 Orr-Weaver TL, Szostak JW, Rothstein RJ. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 1981;78(10):6354-6358.
5 Carlson M, Botstein D. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms
of yeast invertase. Cell 1982; 28(1): 145-154.
6 Rose MD, Novick P, Thomas JH, Botstein D, Fink GR. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing
shuttle vector. Gene 1987; 60(2-3):237-243.
7 Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in
Saccharomyces cerevisiae. Genetics 1989; 122(1): 19-27.
8 Law RHP, Rowley MJ, Mackay IR, Corner B. Expression in Saccharomyces cerevisiae of antigenically and enzy-matically active
recombinant glutamuic acid decarboxylase. J. of Biotechnology 1998; 61:57-68.
9 Bitter GA, Egan KM. Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate
dehydrogenase gene promoter. Gene. 1984; 32(3):263-74.