Expression of Recombinant Proteins in Yeast - - BioPharm International

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Expression of Recombinant Proteins in Yeast

Other Yeast Systems


Table 3. Other yeast expression systems
A few other fungal systems have also been successfully used for recombinant protein production. Some of these systems are based on non-fermentation yeast species like Pichia methanolica and Hansenula polymorpha. These are similar to Pichia pastoris and can be grown to very high cell densities and high-level protein expression has been achieved. There is also a wide range of expression systems similar to the Pichia and Saccharomyces system available for recombinant protein expression. Table 3 shows some available expression technologies for these yeast systems.

This article is an excerpt from the chapter, "Advances in Improved Expression of Recombinant Proteins in Microbial Systems," in Eric S. Langer, Ed., Advances in Large-Scale Biopharmaceutical Manufacturing and Scale-Up Production (ASM Press, 2004).

Daniel Rudolph, Ph.D., is a process engineer in the process development group of Cambrex Bio Science Baltimore, Inc., 5901 E. Lombard Street, Baltimore, MD 21224, 410.563.9200,
. Co-authors Sriram Srinivasan, Ph.D., Don R. Durham, Ph.D., and Aaron Heifetz, Ph.D., previously served at Cambrex in the roles of process engineer, upstream process development group; vice president of technology; and vice president, general manager, and site director, respectively.

References

1. Rudolph D, Srinivasan S, Durham DR, Heifetz A. Advances in improved ex-pression of recombinant proteins in microbial systems. In: Langer ES, editor. Advances in large-scale biopharm-aceutical manufacturing and scale-up production. Volume 1: emerging tech-nologies and scientific advancements. Washington DC: ASM Press; 2004 October. p. 194-228.

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