Applying Fusion Protein Technology to E. Coli - - BioPharm International

ADVERTISEMENT

Applying Fusion Protein Technology to E. Coli


BioPharm International


51. Koken MH, Odijk HH, van Duin M, Fornerod M, Hoeijmakers JH. Augmentation of protein production by a combination of the T7 RNA polymerase system and ubiquitin fusion: overproduction of the human DNA repair protein, ERCC1, as an ubiquitin fusion protein in Escherichia coli. Biochem. Biophys. Res. Commun. 1993; 195:643-653.

52. Murby M, Cedergren L, Nilsson J, Nygren PA, Hammarberg B, Nilsson B, et al. Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy. Biotechnol. Appl. Biochem. 1991; 14:336-346.

53. Shen SH. Multiple joined genes prevent product degradation in Escherichia coli. Proc. Natl. Acad. Sci. 1984; 81:4627-31.

54. Varshavsky A. The N-end rule: functions, mysteries, uses. Proc. Natl. Acad. Sci. 1996; 93:12142-9.

55. Kishi A, Nakamura T, Nishio Y, Maegawa H, Kashiwagi A. Sumoylation of Pdx1 is associated with its nuclear localization and insulin gene activation. Am. J. Physiol. Endocrinol. Metab. 2003; 284:E830-40.

56. Nikaido H. Maltose transport system of Escherichia coli: an ABC-type transporter. FEBS Lett. 1994; 346:55-8.

57. Balbas P. Understanding the art of producing protein and nonprotein molecules in Escherichia coli. Mol. Biotech. 2001; 19:251-267.

58. Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene. 1997; 192:277-281.

59. Jenny RJ, Mann KG, Lundblad RL. A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Protein Express. Purif. 2003; 31:1-11.

60. Carrington JC, Cary SM, Parks TD, Dougherty WG. A second proteinase encoded by a plant potyvirus genome. Embo. J. 1989; 8:365-70.

61. Baneyx F. Recombinant protein expression in Escherichia coli. Opin. Biotech. 10: 411-421.


blog comments powered by Disqus

ADVERTISEMENT

Moscow Hosts IFPMA Biosimilars Conference
May 17, 2013
AbbVie and Alvine Will Collaborate on Celiac Disease Therapy
May 15, 2013
FDA Issues Pharmacoepidemiologic Safety Study Guidance
May 14, 2013
USP Launches Initiative to Fight Counterfeit Drugs in Sub-Saharan Africa
May 13, 2013
Amgen Forms New Joint Venture to Commercialize Vectibix in China
May 13, 2013
Upcoming Conferences
UPCOMING CONFERENCES

Access Programs for Investigational and Pre-Launch Drugs
Philadelphia, PA | July 17-18, 2013
Request Brochure

Strategic Pipeline Planning & Portfolio Valuation
Philadelphia, PA | August 13-14, 2013
Request Brochure

MES 2013 - Forum on Manufacturing Execution Systems
Philadelphia, PA | August 14-15, 2013
Request Brochure

Mobile Innovation for the Life Sciences Industry
Philadelphia, PA | August 20-21, 2013
Request Brochure

See All Conferences >>

ADVERTISEMENT

Author Guidelines
FindPharma
Source: BioPharm International,
Click here