Applying Fusion Protein Technology to E. Coli - - BioPharm International

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Applying Fusion Protein Technology to E. Coli


BioPharm International


51. Koken MH, Odijk HH, van Duin M, Fornerod M, Hoeijmakers JH. Augmentation of protein production by a combination of the T7 RNA polymerase system and ubiquitin fusion: overproduction of the human DNA repair protein, ERCC1, as an ubiquitin fusion protein in Escherichia coli. Biochem. Biophys. Res. Commun. 1993; 195:643-653.

52. Murby M, Cedergren L, Nilsson J, Nygren PA, Hammarberg B, Nilsson B, et al. Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy. Biotechnol. Appl. Biochem. 1991; 14:336-346.

53. Shen SH. Multiple joined genes prevent product degradation in Escherichia coli. Proc. Natl. Acad. Sci. 1984; 81:4627-31.

54. Varshavsky A. The N-end rule: functions, mysteries, uses. Proc. Natl. Acad. Sci. 1996; 93:12142-9.

55. Kishi A, Nakamura T, Nishio Y, Maegawa H, Kashiwagi A. Sumoylation of Pdx1 is associated with its nuclear localization and insulin gene activation. Am. J. Physiol. Endocrinol. Metab. 2003; 284:E830-40.

56. Nikaido H. Maltose transport system of Escherichia coli: an ABC-type transporter. FEBS Lett. 1994; 346:55-8.

57. Balbas P. Understanding the art of producing protein and nonprotein molecules in Escherichia coli. Mol. Biotech. 2001; 19:251-267.

58. Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene. 1997; 192:277-281.

59. Jenny RJ, Mann KG, Lundblad RL. A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Protein Express. Purif. 2003; 31:1-11.

60. Carrington JC, Cary SM, Parks TD, Dougherty WG. A second proteinase encoded by a plant potyvirus genome. Embo. J. 1989; 8:365-70.

61. Baneyx F. Recombinant protein expression in Escherichia coli. Opin. Biotech. 10: 411-421.


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