Efficiency Measurements for Chromatography Columns - Using the method of moments provides a better characterization of column effluent curves than the frequently used Gaussian approximation. -

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Efficiency Measurements for Chromatography Columns
Using the method of moments provides a better characterization of column effluent curves than the frequently used Gaussian approximation.


BioPharm International
Volume 18, Issue 8


Nomenclature
The two distributions contain the same information, but the curve for the differentiated step is more easily obtained. The situation for non-linear distributions is more complicated, but again the differential curve is generally more useful.

In summary, the method of moments when applied to the reverse-flow technique allows for a non-intrusive and accurate method to determine the quality of flow distribution. This is particularly useful for preparatory or commercial columns, where column packing is a tedious and expensive task. The use of statistical moments to evaluate column performance can provide a great deal of information on column performance with little effort.

Anurag Rathore is a principal scientist at Amgen Inc., 30W-2-A, One Amgen Center Drive, Thousand Oaks, CA 91320, 805.447.4491, fax 805.499.5008,
.
John Moscariello is a scientist at Amgen Inc., Seattle, WA.
Edwin Lightfoot is a professor at Department of Chemical and Biological Engineering, University of Wisconsin, Madison.

REFERENCES:

1. Martin AJP, Synge ELM. A new form of chromatogram employing two liquid phases. I. A theory of chrom-atography. 2 Application of the micro-determination of the higher monoamino-acids in proteins. Biochem. J. 1941; 35:1358.

2. Giddings JC, Eyring H. A molecular dynamic theory of chromatography. J. Phys. Chem. 1955; 59:416.

3. Giddings JC. The random downstream migration of molecules in chromatography. J. Chem. Ed. 1958; 35:588.

4. ASTM, March 1992, E 682.

5. Coffman JL. Protein diffusion in chromatographic media. doctoral thesis, U. Wisconsin. 1994.

6. Horváth CS, Melander WR, in Heftmann E (ed.). Theory of chromatography. Chromatography: fundamentals and applications in chromatographic and electropho-retic methods. Part A: Fund-amentals and techniques. New York, Elsevier Scientific; 1983:27.

7. Giddings JC. Generation of variance, theoretical plates, resolution, and peak capacity in electrophoresis and sedimen-tation. Sep. Sci. 1969; 4:476.

8. Giddings JC. Dynamics of chromatography. New York, Marcel Dekker 1965.

9. Lightfoot EN, Coffman JL, Lode F, Yuan QS, Perkins TW, Root TW. Refining the scale-up of chromatographic separations. J. Chrom A. 1997; 760: 139.

10. Bird, RB, Stewart WE, Lightfoot EN. Transport Phenomena. New York, Wiley; 2002.

11. Scheinder P, Smith JM. Adsorption rate constant form chroma-tography. AICHE J. 1968; 14:762.

12. Moscariello JS, Purdom G, Coffman JL, Root TW, Lightfoot EN. Characterizing the performance of industrial-scale columns. J. Chrom. A. 2001; 908:131.

13. Hill CG. An introduction to chemical engineering kinetics and reactor design. New York, Wiley; 1977.


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