Efficiency Measurements for Chromatography Columns - Using the method of moments provides a better characterization of column effluent curves than the frequently used Gaussian approximation. -

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Efficiency Measurements for Chromatography Columns
Using the method of moments provides a better characterization of column effluent curves than the frequently used Gaussian approximation.


BioPharm International
Volume 18, Issue 8


Nomenclature
The two distributions contain the same information, but the curve for the differentiated step is more easily obtained. The situation for non-linear distributions is more complicated, but again the differential curve is generally more useful.

In summary, the method of moments when applied to the reverse-flow technique allows for a non-intrusive and accurate method to determine the quality of flow distribution. This is particularly useful for preparatory or commercial columns, where column packing is a tedious and expensive task. The use of statistical moments to evaluate column performance can provide a great deal of information on column performance with little effort.

Anurag Rathore is a principal scientist at Amgen Inc., 30W-2-A, One Amgen Center Drive, Thousand Oaks, CA 91320, 805.447.4491, fax 805.499.5008,
.
John Moscariello is a scientist at Amgen Inc., Seattle, WA.
Edwin Lightfoot is a professor at Department of Chemical and Biological Engineering, University of Wisconsin, Madison.

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4. ASTM, March 1992, E 682.

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9. Lightfoot EN, Coffman JL, Lode F, Yuan QS, Perkins TW, Root TW. Refining the scale-up of chromatographic separations. J. Chrom A. 1997; 760: 139.

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