Jul 11, 2006 By:
Laura Bush BioPharm International
Volume 19,
Issue 7
Removing the analytical SDS-PAGE
bottleneck in Protein Expression
The need for pure and correctly folded target proteins is a key driver
for parallel protein expression and purification. Protein expression
studies can easily lead to large numbers of samples that have to be
handled and analyzed. Samples out of a single protein target experiment
in pharmaceutical research often get multiplied by using several
constructs, different tags and cell lines, various time points or
temperatures. As a result several hundreds of samples traditionally are
run by one dimensional SDS-PAGE. These slab gel measurements are slow
and time consuming, additionally the reproducibility is not really
impressing and quantitation needs to be done separately e.g. by BCA or Bradford assay.
Automated and miniaturized analysis on a "Lab-on-a-Chip" based system
enables investigators to conduct such experiments quicker with less
efforts.
For more information, please contact Laura Bush at lbush@advanstar.com
or see our website at www.biopharminternational.com
