Discovery Pipeline: Topical Delivery of siRNA - A team from Northwestern University has demonstrated the feasibility of topical delivery of small interfering RNA (siRNA). - BioPharm International

ADVERTISEMENT

Discovery Pipeline: Topical Delivery of siRNA
A team from Northwestern University has demonstrated the feasibility of topical delivery of small interfering RNA (siRNA).


BioPharm International
Volume 25, Issue 8, pp. 8

A team from Northwestern University has demonstrated the feasibility of topical delivery of small interfering RNA (siRNA) in a July 2012 publication online in the Proceedings of the National Academy of Sciences. To date, the biggest hurdle in developing (siRNA) therapeutics to effect gene knockdown in vivo has been designing an effective delivery system. While lipid nanoparticle delivery systems have shown some success, use of this platform has been confined to targets in the liver, where the lipid nanoparticles are most efficiently taken up. Targeting other tissues has been more difficult.

To get siRNA to penetrate the skin, the researchers used a delivery system where siRNA strands were arranged symmetrically around a gold nanoparticle and covalently immobilized to form a spherical structure. In this arrangement, the siRNAs are densely packed and highly oriented, and the particles are readily taken up by skin cells. The spherical nucleic acid nanoparticle conjugates could be delivered in saline to cultured cells or suspended in a commercial moisturizer for delivery to the skin. The authors created a spherical nucleic acid nanoparticle conjugate containing siRNA against epidermal growth factor receptor (EGFR), and demonstrated gene knockdown in cultured keratinocytes, human epidermis, and in the skin of nude mice. When mice were treated topically with 1.5 μM EGFR siRNA for 3 weeks, EGFR expression was almost eliminated and skin thickness was reduced by 40% with no histological or clinical signs of toxicity. An effective topical delivery system would pave the way for developing siRNA therapies against such disorders as skin carcinomas, psoriasis, or to promote wound healing.

Source: D. Zheng et al., Proc. Natl. Acad. Sci. online DOI: 10.1073/pnas.1118425109, Jul. 6, 2012.

blog comments powered by Disqus

ADVERTISEMENT

ADVERTISEMENT

Novartis Reports Positive Results for Secukinumab in Ankylosing Spondylitis Trials
October 23, 2014
Pall ForteBio Releases Bioprocessing Contamination Detection Kit
October 22, 2014
Roche to Expand and Improve its Basel Site
October 22, 2014
FDA Panel Unanimously Backs Secukinumab for the Treatment of Psoriasis
October 22, 2014
EMA Works to Speed Up Ebola Treatment
October 20, 2014
Author Guidelines
Source: BioPharm International,
Click here