SEC-MALS Detector for UHPLC - - BioPharm International

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SEC-MALS Detector for UHPLC


BioPharm International
Volume 27, Issue 6, pp. 48

Wyatt Technology offers the µDAWN, a multi-angle light scattering (MALS) detector that can be attached to any UHPLC system to determine molecular weights and sizes of polymers, peptides, and proteins or other biopolymers directly, without resorting to column calibration or reference standards. The µDAWN detector connects to the Optilab UT-rEX, a refractive index detector for UHPLC.

Wyatt reduced the conventional light scattering flow cell volume from 63 µL to less than 10µL to accommodate the narrow peaks produced by UHPLC seperation. By minimizing interdetector mixing, the band broadening between the µDAWN MALS and Optilab UT-rEX detectors is under 7 µL, while the band broadening between the UHPLC’s UV detector and the µDAWN detector is 2 µL. As a result of the reduced cell volume and interdetector band broadening, the µDAWN/UT-rEX system can accurately analyze the molar mass and size of UHPLC peaks without loss of resolution. In addition, the WyattQELS Dynamic Light Scattering (DLS) module measures DLS in the µDAWN flow cell simultaneously with MALS acquisition, quickly computing hydrodynamic radii.

Wyatt Technology

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