SEC-MALS Detector for UHPLC - - BioPharm International

ADVERTISEMENT

SEC-MALS Detector for UHPLC


BioPharm International
Volume 27, Issue 6, pp. 48

Wyatt Technology offers the µDAWN, a multi-angle light scattering (MALS) detector that can be attached to any UHPLC system to determine molecular weights and sizes of polymers, peptides, and proteins or other biopolymers directly, without resorting to column calibration or reference standards. The µDAWN detector connects to the Optilab UT-rEX, a refractive index detector for UHPLC.

Wyatt reduced the conventional light scattering flow cell volume from 63 µL to less than 10µL to accommodate the narrow peaks produced by UHPLC seperation. By minimizing interdetector mixing, the band broadening between the µDAWN MALS and Optilab UT-rEX detectors is under 7 µL, while the band broadening between the UHPLC’s UV detector and the µDAWN detector is 2 µL. As a result of the reduced cell volume and interdetector band broadening, the µDAWN/UT-rEX system can accurately analyze the molar mass and size of UHPLC peaks without loss of resolution. In addition, the WyattQELS Dynamic Light Scattering (DLS) module measures DLS in the µDAWN flow cell simultaneously with MALS acquisition, quickly computing hydrodynamic radii.

Wyatt Technology

blog comments powered by Disqus

ADVERTISEMENT

ADVERTISEMENT

Bristol-Myers Squibb and Five Prime Therapeutics Collaborate on Development of Immunomodulator
November 26, 2014
Merck Enters into Licensing Agreement with NewLink for Investigational Ebola Vaccine
November 25, 2014
FDA Extends Review of Novartis' Investigational Compound for Multiple Myeloma
November 25, 2014
AstraZeneca Expands Biologics Manufacturing in Maryland
November 25, 2014
GSK Leads Big Pharma in Making Its Medicines Accessible
November 24, 2014
Author Guidelines
Source: BioPharm International,
Click here