http://www.biopharminternational.com/biopharm/Upstream+Processing/Article/list/41918 Protein expression, fermentation & cell culture, and plant & animal expression methods, along with process development, scale up, and technology transfer for upstream steps. 2013-05-23T08:00:58Z http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Process-Development-and-Spiking-Studies-for-Virus-/ArticleStandard/Article/detail/813389?contextCategoryId=41918&ref=25 This study on a recombinant human follicle stimulating hormone demonstrates the use of virus filters to reduce the risk of contamination. 2013-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/202013/813389/PeerRevButton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/202013/813389/Peer-Review-Figure-2_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Disposables/Making-the-Best-Choices-in-Single-Use-Tubing/ArticleStandard/Article/detail/810532?contextCategoryId=41918&ref=25 Jerold Martin considers the types of tubing available to the industry and how to make an informed selection. 2013-04-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/152013/810532null XML http://www.biopharminternational.com/biopharm/Good+Manufacturing+Practices/Growth-Kinetics-of-Human-Mesenchymal-Stem-Cells-in/ArticleStandard/Article/detail/810545?contextCategoryId=41918&ref=25 The authors describe the growth characteristics of human mesenchymal stem cells cultured in a stirred-tank bioreactor. 2013-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/152013/810545null XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Milestones-and-Moderate-Progress-in-2012-Drug-Appr/ArticleStandard/Article/detail/810538?contextCategoryId=41918&ref=25 First gene therapy and plant-based expression vector products approved in 2012. 2013-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/152013/810538null XML http://www.biopharminternational.com/biopharm/Basic+Training/ZFN-Technology-Advances-Biopharmaceutical-Manufact/ArticleStandard/Article/detail/810535?contextCategoryId=41918&ref=25 Applications of ZFN technology in biopharmaceutical cell-line engineering. 2013-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/152013/810535null XML http://www.biopharminternational.com/biopharm/Analytical+Methods+Articles/Overcoming-Challenges-in-the-Reconstitution-of-a-H/ArticleStandard/Article/detail/809073?contextCategoryId=41918&ref=25 The authors present approaches used to reduce reconstitution time of a lyophilized high-concentration protein drug product. 2013-03-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142013/809073/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142013/809073/Peer-In-Figure-1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Therapeutic-Antibodies-in-Review/ArticleStandard/Article/detail/804370?contextCategoryId=41918&ref=25 Innovative products and a range of indications drive the therapeutic antibody market. 2013-02-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/052013/804370/BP0213_121528350_web_190x210_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/The-Vaccine-Scene/ArticleStandard/Article/detail/804362?contextCategoryId=41918&ref=25 A look at vaccine history, markets, manufacturing, and overcoming the scale-up dilemma. 2013-02-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/052013/804362/BP0213_Vaccines-ATMI-Figure-1_web_190x210_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Viral-Vaccine-Manufacturing/ArticleStandard/Article/detail/804363?contextCategoryId=41918&ref=25 Tony Hitchcock of Cobra Biologics discusses challenges posed by production of viral vectors for vaccines. 2013-02-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/052013/804363/BP0213_001_Cover(without-lines-&-text)_web_190x210_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Bioconjugates-The-Adaptable-Challenge/ArticleStandard/Article/detail/802146?contextCategoryId=41918&ref=25 Conjugation of a biologic to a carrier molecule can solve problems in solubility and stability, but introduces its own challenges. 2013-01-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/022013/802146/BP0113_Bioconjugates-storyTable1_web__flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/The-Future-of-Biopharma/ArticleStandard/Article/detail/802143?contextCategoryId=41918&ref=25 What the industry's future holds and what needs to be done to get there. 2013-01-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/022013/802143/BP0113_bp0113_136120148-1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/Upstream-Downstream-Processing-in-Motion/ArticleStandard/Article/detail/796141?contextCategoryId=41918&ref=25 GE Healthcare Life Sciences' ReadyToProcess platform aims to streamline bioprocessing. 2012-11-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452012/796141/GEHealthcareRTR_Interior_Web_flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/A-25-Year-Retrospective-on-Protein-Recovery-throug/ArticleStandard/Article/detail/796157?contextCategoryId=41918&ref=25 This month, Jerold Martin of Pall Life Sciences takes a look at protein recovery through direct-flow microporous membrane filters over the past 25 years. 2012-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452012/796157null XML http://www.biopharminternational.com/biopharm/Quality/EU-Sets-Guidelines-for-Biosimilar-mAbs/ArticleStandard/Article/detail/796152?contextCategoryId=41918&ref=25 The European Medicines Agency has added granularity to its biosimilars approval pathway by releasing a guideline on mAbs. 2012-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452012/796152/dv321052_3_cmyk_Web_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Evaluation-of-Single-Use-Fluidized-Bed-Centrifuge-/ArticleStandard/Article/detail/796144?contextCategoryId=41918&ref=25 This article discusses the evaluation of a novel single-use fluidized bed centrifuge for harvesting of antibodies. 2012-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452012/796144/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452012/796144/Chamber-Image_Web_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Considerations-for-Scale-Up-of-Stem-Cell-Cultures/ArticleStandard/Article/detail/796156?contextCategoryId=41918&ref=25 Scaling up stem-cell cultures requires careful consideration of the bioreactor design. 2012-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452012/796156/ThinkStock_106522811_Web_flash.jpg XML http://www.biopharminternational.com/biopharm/Outsourcing/A-Manufacturing-Capacity-Sharing-Model/ArticleStandard/Article/detail/791289?contextCategoryId=41918&ref=25 The manufacturing capacity-sharing model between Merck and MedImmune ushers in a new paradigm of "co-opetition." 2012-10-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/402012/791289null XML http://www.biopharminternational.com/biopharm/Quality/Key-Considerations-in-Biosimilars-Development/ArticleStandard/Article/detail/791275?contextCategoryId=41918&ref=25 Understanding opportunities and challenges across all major phases of development. 2012-10-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/402012/791275/Biosimilars-Figue-1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Bringing-Innovation-to-Neglected-Disease-RampD/ArticleStandard/Article/detail/791272?contextCategoryId=41918&ref=25 A new report highlights the industry's contributions to neglected diseases and calls for further collaboration. 2012-10-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/402012/791272/BioventuresNEW_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Correlating-Product-Lifecycle-and-Manufacturing-Si/ArticleStandard/Article/detail/791288?contextCategoryId=41918&ref=25 MIT survey results address product and site characteristics that statistically correlate with quality performance. 2012-10-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/402012/791288/Figure3_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Basic+Training/Upstream-Processing-A-Primer/ArticleStandard/Article/detail/787747?contextCategoryId=41918&ref=25 NIBRT's Ian Nelligan on what to expect when starting an upstream process, including the choice between single-use and stainless-steel bioreactors. 2012-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362012/787747null XML http://www.biopharminternational.com/biopharm/Downstream+Processing/A-25-Year-Retrospective-on-Bioprocessing-mAbs/ArticleStandard/Article/detail/787749?contextCategoryId=41918&ref=25 Michiel E. Ultee of Laureate Biopharmaceutical Services gives an update on 1988 article regarding virus testing and how the advance of monoclonal antibodies has changed processes. 2012-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362012/787749null XML http://www.biopharminternational.com/biopharm/Quality/Bucking-the-Trend/ArticleStandard/Article/detail/787741?contextCategoryId=41918&ref=25 A perspective on why platform processes are not and should not define the future of bioprocess development efforts. This article is part of a special section on biopharmaceutical trends. 2012-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362012/787741null XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/A-Method-for-Removal-of-Endotoxin-from-Pharmaceuti/ArticleStandard/Article/detail/787744?contextCategoryId=41918&ref=25 The authors describe a simple method to remove endotoxins from highly viscous formulations. 2012-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362012/787744/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362012/787744/BP0912_28_Hagelin_Figure-1_flash.jpg XML http://www.biopharminternational.com/biopharm/Basic+Training/Best-Quality-Practices-for-Biomedical-RampD/ArticleStandard/Article/detail/784474?contextCategoryId=41918&ref=25 Members from an ASQ working group provide analytical methods to enable PAT. 2012-08-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/322012/784474/BP0812_050_BootCamp_Trotter_Figure2Rev_thumbnail.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/322012/784474/test_flash.gif XML http://www.biopharminternational.com/biopharm/Basic+Training/Considerations-for-Successful-Upstream-Process-Dev/ArticleStandard/Article/detail/781177?contextCategoryId=41918&ref=25 Selection of the right cell line, culture medium, and bioreactor conditions is key to setting up the upstream portion of the biopharmaceutical manufacturing process. 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781177null XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/A-25-Year-Retrospective-on-Bio-Engineering/ArticleStandard/Article/detail/781175?contextCategoryId=41918&ref=25 Steven S. Kuwahara, PhD, principal consultant at GXP BioTechnology LLC, gives an update on "Engineering the Cell-System Interface." 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781175null XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/A-New-Era-in-Cell-Culture-Media-Development/ArticleStandard/Article/detail/781184?contextCategoryId=41918&ref=25 Cell-line specific complex media supplements combine chemically defined media additives into a single supplement. 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781184/BP0712_Sheffield-Media-Figure-1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Optimizing-Expression-Systems/ArticleStandard/Article/detail/781181?contextCategoryId=41918&ref=25 Industry experts discuss methods for optimizing protein expression in bacterial and mammalian cell lines. 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781181/BP0712_BI-Hex-Figure1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/Biomanufacturing-Innovation/ArticleStandard/Article/detail/781182?contextCategoryId=41918&ref=25 Industry wants more innovation, but can suppliers meet customers' needs? 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781182null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Scale-up-of-Fed-Batch-Culture-to-Produce-Plasmid-D/ArticleStandard/Article/detail/781176?contextCategoryId=41918&ref=25 The authors present scale-up from a 5-L fermentor to a 50-L pilot-scale using the criterion of constant power consumption per unit liquid volume. 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781176/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781176/BP0712_Hernandez-Figurre-1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Allogeneic-Versus-Autologous-Stem-Cell-Therapy/ArticleStandard/Article/detail/781178?contextCategoryId=41918&ref=25 The author discusses potential manufacturing costs & challenges of allogeneic & autologous stem-cell therapy. 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781178/BP0712_Malik-Figure-1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Supplementary-Material-for-Allogeneic-Versus-Autol/ArticleStandard/Article/detail/781183?contextCategoryId=41918&ref=25 This article contains online-exclusive supplemental material for Malik's article entitled, "Allogenic Versus Autologous Stem-Cell Therapy." 2012-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/282012/781183null XML http://www.biopharminternational.com/biopharm/Quality/25-Years-of-BioPharma-Industry-Growth/ArticleStandard/Article/detail/775160?contextCategoryId=41918&ref=25 A review of key industry shifts and promises for the future. 2012-06-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/212012/775160/BP0612_21_140654677-2-Duotone_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Developing-Discoveries-into-New-Therapeutics/ArticleStandard/Article/detail/775169?contextCategoryId=41918&ref=25 Formulators and developers are at the heart of the industry's basic premise—they are saving lives. 2012-06-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/212012/775169null XML http://www.biopharminternational.com/biopharm/Quality/25-Years-of-Biopharmaceutical-Innovation-An-Expert/ArticleStandard/Article/detail/775157?contextCategoryId=41918&ref=25 Industry experts discuss significant achievements. Plus: What's in store for the future. 2012-06-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/212012/775157/BP0612_54_Covers-1988_Jan_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/25-Years-of-Protein-Expression/ArticleStandard/Article/detail/775159?contextCategoryId=41918&ref=25 Charles H. Squires of Pfenex discusses advances in expression platform solutions. 2012-06-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/212012/775159null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/New-Biopharmaceuticals/ArticleStandard/Article/detail/775167?contextCategoryId=41918&ref=25 A review of new biologic drug approvals over the years, featuring highlights from 2010 and 2011. 2012-06-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/212012/775167null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Choosing-an-Expression-System/ArticleStandard/Article/detail/771923?contextCategoryId=41918&ref=25 A Q&A with Alan Shaw of Vaxinnate. This article is part of a special section on expression systems. 2012-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182012/771923null XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Consistent-Production-of-Genetically-Stable-Mammal/ArticleStandard/Article/detail/771927?contextCategoryId=41918&ref=25 The author describes expression technology that produces cell lines with high genetic stability. 2012-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182012/771927/BP0512_57_Catalent_Figure-1_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Oxidative-Folding-of-Proteins-in-iEscherichia-Coli/ArticleStandard/Article/detail/771918?contextCategoryId=41918&ref=25 The author highlights novel strains and methods that have recently been shown to express multidisulfide bonded proteins. 2012-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182012/771918/BP0512_50_NewEngEColi_Figure-2_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Development-of-an-Alternative-Monoclonal-Antibody-/ArticleStandard/Article/detail/771924?contextCategoryId=41918&ref=25 MAb polishing using salt tolerant interaction membrane chromatography. 2012-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182012/771924/BP0512_38_Kang-Figure-1a_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Optimizing-Drug-Delivery-for-Modern-Biologics/ArticleStandard/Article/detail/771928?contextCategoryId=41918&ref=25 This article discusses potential opportunities to improve the patient experience through formulation and delivery device technologies. 2012-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182012/771928null XML http://www.biopharminternational.com/biopharm/Downstream+Processing/Single-Use-Redundant-Filtration/ArticleStandard/Article/detail/767896?contextCategoryId=41918&ref=25 The authors describe a new assembly for bulk and final drug product filling operations. 2012-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142012/767896/BP0412_113477336_flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/Optimizing-Adjuvant-Filtration/ArticleStandard/Article/detail/767889?contextCategoryId=41918&ref=25 A technical rountable featuring Sartorius Stedim Biotech, Pall Life Sciences, 3M Purification, Asahi Kasei Bioprocess, and Bio-Rad Laboratories. 2012-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142012/767889/BP0412_Sartorius_flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/Pointed-Debate/ArticleStandard/Article/detail/767891?contextCategoryId=41918&ref=25 Industry experts discuss the benefits and challenges of self-administration of injectable therapies. 2012-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142012/767891/BP0412_82047762_Cvr-St_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Scale-up-of-Human-Mesenchymal-Stem-Cells-on-Microc/ArticleStandard/Article/detail/763573?contextCategoryId=41918&ref=25 Demonstration of large-scale stem-cell scale-up. 2012-03-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/102012/763573/BP0312_28_Kehoe_Opening-Art_flash.jpg XML http://www.biopharminternational.com/biopharm/Downstream+Processing/Recombinant-Albumin-Facilitates-Formulation-Design/ArticleStandard/Article/detail/763570?contextCategoryId=41918&ref=25 Recombinant albumin can stabilize a drug product and assist in API release. 2012-03-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/102012/763570/BP0312_41_Sharpe_Figure-1_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/A-25-Year-Retrospective-on-Mammalian-Cell-Culture/ArticleStandard/Article/detail/757941?contextCategoryId=41918&ref=25 This month, we revisit "Industrial-Scale Mammalian Cell Culture, Part I: Bioreactor Design Considerations." 2012-02-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/062012/757941null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/The-Conception-and-Production-of-Conjugate-Vaccine/ArticleStandard/Article/detail/754435?contextCategoryId=41918&ref=25 Recombinant technology can be used to produce conjugate vaccines. 2012-01-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/532011/754435/BP0112_25_95468876_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality%2FGMP%2FValidation++Whitepapers/The-Employment-Outlook-Brightens/ArticleStandard/Article/detail/750859?contextCategoryId=41918&ref=25 Readers react to the economic turmoil of the past year and look longingly forward to 2012. 2011-12-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/492011/750859/BP1211_22_103772148_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Disposables/Emerging-Bioprocessing-Methods/ArticleStandard/Article/detail/746268?contextCategoryId=41918&ref=25 New technology is designed to improve production efficiency by taking advantage of the properties of single-use bags. 2011-11-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/442011/746268/BP1111_Niazi_Figure1_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Biobetters-and-the-Future-Biologics-Market/ArticleStandard/Article/detail/746281?contextCategoryId=41918&ref=25 Despite their difficult approval pathway, biobetters offer the potential for innovation and decreased healthcare costs. 2011-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/442011/746281null XML http://www.biopharminternational.com/biopharm/Quality/DNA-Vaccine-Delivery/ArticleStandard/Article/detail/742599?contextCategoryId=41918&ref=25 Development of the ideal DNA vaccine requires the optimization of delivery strategies and plasmid vectors. 2011-10-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/412011/742599/BPsupp1011_Cobra-ORT-VAC-New-Figure-2_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Challenges-and-Trends-in-Vaccine-Manufacturing/ArticleStandard/Article/detail/742600?contextCategoryId=41918&ref=25 An evaluation of the technologies needed to develop a safe, effective, and economically efficient vaccine. This article is part of a special section on vaccines. 2011-10-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/412011/742600/BPsupp1011_Cv1_v3r2_high_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Perspectives-in-MicroRNA-Therapeutics/ArticleStandard/Article/detail/742606?contextCategoryId=41918&ref=25 The authors provide insight into microRNA biology, and the simplicity of anti-miR oligonucleotide drug delivery. 2011-10-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/412011/742606/BP1011_Perspectives-MicroRNA-Figure-3_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Contract-Services-Spending-Whos-Spending-What-and-/ArticleStandard/Article/detail/742603?contextCategoryId=41918&ref=25 A survey provides insight into drug companies' plans for spending on outsourced services. This article contains bonus online material. 2011-10-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/412011/742603null XML http://www.biopharminternational.com/biopharm/Operations/Organizational-Structures-of-Process-Development-a/ArticleStandard/Article/detail/737331?contextCategoryId=41918&ref=25 How to strike a balance between site autonomy and global coordination. 2011-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/352011/737331/PeerRevButton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/352011/737331/BP0911_Konstantinov-Figure-2_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Feature+Rotating+Article/Fermentation-Process-Technology-Transfer-for-Produ/ArticleStandard/Article/detail/728236?contextCategoryId=41918&ref=25 The authors describe challenges faced in transfer and scale-up of a fermentation process. 2011-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/262011/728236/BP_0711_Sharma-Art_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/The-Use-of-the-ATF-System-to-Culture-Chinese-Hamst/ArticleStandard/Article/detail/724371?contextCategoryId=41918&ref=25 Cell concentrations and resulting protein concentrations are higher in a concentrated fed-batch process than in a standard fed-batch culture system. 2011-06-01T04:00:00Z XML http://www.biopharminternational.com/biopharm/Outsourcing/Better-Upstream-Technologies/ArticleStandard/Article/detail/724370?contextCategoryId=41918&ref=25 A recent industry survey shows keen interest in improving bioreactors and cell-culture media. 2011-06-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/222011/724370null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Plants-As-an-Innovative-and-Accelerated-Vaccine-Ma/ArticleStandard/Article/detail/718082?contextCategoryId=41918&ref=25 A plant-based expression system could provide greater speed and capacity at a comparatively low cost. 2011-05-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182011/718082null XML http://www.biopharminternational.com/biopharm/Disposables/Increasing-Efficiency-in-Protein-and-Cell-Producti/ArticleStandard/Article/detail/718080?contextCategoryId=41918&ref=25 An off-the-shelf, single-use perfusion system. 2011-05-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182011/718080/bp0511_Sartorius.web_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/The-Potential-Application-of-Hollow-Fiber-Bioreact/ArticleStandard/Article/detail/718081?contextCategoryId=41918&ref=25 A hollow fiber matrix allows for efficient harvest of secreted proteins. 2011-05-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182011/718081/bp0511_Fibercell_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Application-of-Ion-Chromatography-with-Electrochem/ArticleStandard/Article/detail/718083?contextCategoryId=41918&ref=25 An overview of applications in the analysis of nutrients and metabolites. 2011-05-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182011/718083null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Bioengineered-Protein-A-Polymer-Beads-for-High-Aff/ArticleStandard/Article/detail/718079?contextCategoryId=41918&ref=25 The authors discuss an alternative to traditional Protein A resins. 2011-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182011/718079/bp0511_Rehm._web_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/New-Binary-Gas-Integrity-Test-Improves-Membrane-Qu/ArticleStandard/Article/detail/714715?contextCategoryId=41918&ref=25 The authors developed a test for defects in filter membranes. 2011-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142011/714715/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142011/714715/BP0411_Krishnanwebr_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Industrialized-Production-of-Human-iPSC-Derived-Ca/ArticleStandard/Article/detail/711064?contextCategoryId=41918&ref=25 The authors describe an industrialized process for the manufacture of iPSC-derived human cardiomyocytes. 2011-03-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/112011/711064null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/An-Economic-Comparison-of-Three-Cell-Culture-Techn/ArticleStandard/Article/detail/707022?contextCategoryId=41918&ref=25 Comparing the economic feasibility of a typical glycosylated protein. 2011-02-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/072011/707022/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/072011/707022/bp0211_Cover_web_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Comparing-Fed-Batch-Cell-Culture-Performances-of-S/ArticleStandard/Article/detail/703599?contextCategoryId=41918&ref=25 A case study to compare the performances of several types of mixing in disposable bags with stainless steel bioreactors. 2011-01-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/032011/703599/PeerReviewbutton_main.jpg http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/032011/703599null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/High-Throughput-Multi-Product-Liquid-Chromatograph/ArticleStandard/Article/detail/695162?contextCategoryId=41918&ref=25 These new analytical methods can reduce analysis and product development time. 2010-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/462010/695162/bp1110_Rea_flash.jpeg XML http://www.biopharminternational.com/biopharm/Multimedia/Podcast-Answers-to-Your-Questions-about-QbD/ArticleStandard/Article/detail/690731?contextCategoryId=41918&ref=25 Hundreds of listeners tuned into our recent web seminar on Quality by Design, and we didn?t have time to answer all their questions. Fortunately, our speakers gathered to answer the common questions in this podcast. 2010-10-11T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/422010/690731null HTML http://www.biopharminternational.com/biopharm/Quality/Design-Space-Development-for-Lyophilization-Using-/ArticleStandard/Article/detail/685438?contextCategoryId=41918&ref=25 Develop a relevant design space without full factorial DoE. 2010-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362010/685438/bp0910_Sundaram_flash.jpeg XML http://www.biopharminternational.com/biopharm/Sterile+Manufacturing%2FCleaning/Label-Free-and-Labeled-Technology-for-Protein-Char/ArticleStandard/Article/detail/685443?contextCategoryId=41918&ref=25 Use it label-free, or add labels to detect contaminants in solution. 2010-09-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/362010/685443/bp0910_Gerion_flash.jpeg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Rapid-Assessment-of-Molecular-Similarity-between-a/ArticleStandard/Article/detail/683159?contextCategoryId=41918&ref=25 Intact protein LC–MS detected a mass variance of 62 Da and peptide mapping located a difference of two amino acids. 2010-08-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/332010/683159null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Biophysical-Characterization-for-Product-Comparabi/ArticleStandard/Article/detail/683158?contextCategoryId=41918&ref=25 Spectroscopic methods such as circular dichroism can detect subtle differences in higher order structure before and after changes in process and formulation. 2010-08-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/332010/683158null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Using-NMR-Spectroscopy-to-Obtain-the-Higher-Order-/ArticleStandard/Article/detail/683161?contextCategoryId=41918&ref=25 Simple methods can characterize polysaccharide vaccines and recombinant cytokines at high resolution. 2010-08-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/332010/683161null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Capturing-Conformational-Changes-in-Biotherapeutic/ArticleStandard/Article/detail/683160?contextCategoryId=41918&ref=25 By providing information on the relative accessibility of locations within a protein, HDX by mass spectrometry opens new windows into the higher order structure of biomolecules. 2010-08-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/332010/683160null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Protein-Characterization-Through-the-Stages/ArticleStandard/Article/detail/683157?contextCategoryId=41918&ref=25 Biomanufacturers should follow a risk-based approach to decide which methods to use to characterize their products. 2010-08-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/332010/683157null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Development-of-a-Novel-Rolling-Tube-Cell-Culture-P/ArticleStandard/Article/detail/683147?contextCategoryId=41918&ref=25 Rolling-tube system balances scale-up accuracy and thoroughput. 2010-08-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/332010/683147/bp0810_Shimoni_flash.jpeg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/The-Effect-of-Limiting-Phosphate-Using-the-HMS174-/ArticleStandard/Article/detail/677308?contextCategoryId=41918&ref=25 The HMS174 strain, in the absence or presence of excess phosphate, can metabolize acetate efficiently. 2010-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/272010/677308/bp0710_Vedvick_flash.jpeg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Robust-Experimental-Strategies-for-Improving-Upstr/ArticleStandard/Article/detail/677310?contextCategoryId=41918&ref=25 Identify the best experimentation methods for the data you need. 2010-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/272010/677310/bp0710_Snee_flash.jpeg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Podcast-Testing-Fermentation-Equipment/ArticleStandard/Article/detail/673338?contextCategoryId=41918&ref=25 2010-06-08T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/242010/673338null HTML http://www.biopharminternational.com/biopharm/Upstream+Processing/Using-Information-Technology-to-Enhance-Upstream-P/ArticleStandard/Article/detail/673333?contextCategoryId=41918&ref=25 Two case studies show how advanced information technologies make process development more efficient. 2010-06-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/242010/673333null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Partial-Replacement-of-Chemically-Defined-Media-wi/ArticleStandard/Article/detail/673337?contextCategoryId=41918&ref=25 Plant-derived hydrolysates can be used as valuable and practical tools to improve cell culture performance. 2010-06-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/242010/673337null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Developing-an-Automatically-Controlled-Feeding-Pro/ArticleStandard/Article/detail/673330?contextCategoryId=41918&ref=25 An exponential feeding strategy coupled with automation doubled protein yields while reducing fermentation time by 25%. 2010-06-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/242010/673330null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Balancing-Media-Development-with-Patient-Safety/ArticleStandard/Article/detail/673335?contextCategoryId=41918&ref=25 The quest for increased productivity and better process control combined with patient safety has encouraged biopharmaceutical companies to use chemically defined media for cell culture. 2010-06-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/242010/673335null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Best-Practices-for-Microbial-Fermenter-Equipment-C/ArticleStandard/Article/detail/673328?contextCategoryId=41918&ref=25 Wet testing of microbial fermenters allows for a greater understanding of the equipment's capabilities. 2010-06-02T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/242010/673328null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Applying-Computational-Fluid-Dynamics-Technology-i/ArticleStandard/Article/detail/667237?contextCategoryId=41918&ref=25 Computational fluid dynamics can resolve performance problems. 2010-05-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/182010/667237/bp0410_Fang_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Applying-Computational-Fluid-Dynamics-Technology-i/ArticleStandard/Article/detail/663865?contextCategoryId=41918&ref=25 Computational fluid dynamics is a powerful tool to optimize processes. 2010-04-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/142010/663865/bp0410_Fang_flash.jpg XML http://www.biopharminternational.com/biopharm/Quality/Resources/ArticleStandard/Article/detail/654739?contextCategoryId=41918&ref=25 If you are looking for additional information (or terms not included in our glossary), here are some places to begin your search. 2010-02-02T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/062010/654739null XML http://www.biopharminternational.com/biopharm/Quality/Podcast-Series/ArticleStandard/Article/detail/654741?contextCategoryId=41918&ref=25 UPLC, QTof, HDMS, and more: A practical guide to the hottest techniques driving the future of biopharmaceutical science. 2010-02-02T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/062010/654741null XML http://www.biopharminternational.com/biopharm/Quality/Bioterminology/ArticleStandard/Article/detail/654740?contextCategoryId=41918&ref=25 A Guide to the Biopharmaceutical Lexicon, 2010 edition 2010-02-02T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/062010/654740null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Licensing-of-Expression-Systems/ArticleStandard/Article/detail/646887?contextCategoryId=41918&ref=25 Gaining a license can be a complex process, but a few key tips can help you avoid common pitfalls and patent infringements. 2009-12-01T05:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/492009/646887null XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Rapid-Process-Development-for-High-Yield-Plasmid-D/ArticleStandard/Article/detail/640112?contextCategoryId=41918&ref=25 How to reduce plasmid-mediated metabolic burden for higher yields. 2009-11-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/452009/640112/bp1109_manuf_economics_flash.jpg XML http://www.biopharminternational.com/biopharm/Business/Fermentation-and-Cell-Culture/ArticleStandard/Article/detail/627745?contextCategoryId=41918&ref=25 To assess current trends in fermentation and cell culture equipment, BioPharm International turned to Geoff Hodge, managing director of process technology, Xcellerex, Inc.; Günter Jagschies, senior director of strategic customer relations, life sciences, biotechnologies, GE Healthcare; and Rich Mirro, product manager, New Brunswick Scientific. 2009-09-18T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/382009/627745null HTML http://www.biopharminternational.com/biopharm/Upstream+Processing/Avoiding-the-Regulatory-Catch-22/ArticleStandard/Article/detail/615146?contextCategoryId=41918&ref=25 Companies often wait for a critical mass before adopting new technologies. But if no one takes the risk, critical mass will never be reached. 2009-08-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/312009/615146null XML http://www.biopharminternational.com/biopharm/Quality/Analytical-Tools-for-Process-and-Product-Character/ArticleStandard/Article/detail/615148?contextCategoryId=41918&ref=25 Select the best approach to determine critical quality attributes. 2009-08-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/312009/615148/bp0809_analytical_tools_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/Site-Directed-Engineering-of-Defined-Chromosomal-S/ArticleStandard/Article/detail/608684?contextCategoryId=41918&ref=25 Method for integration of transgenes. 2009-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/272009/608684/bp0709_cell_engineering_flash.jpg XML http://www.biopharminternational.com/biopharm/Upstream+Processing/High-Cell-Density-Culture-to-Produce-Plasmid-DNA-f/ArticleStandard/Article/detail/608689?contextCategoryId=41918&ref=25 How to produce Plasmid DNA in a high-cell-density culture. 2009-07-01T04:00:00Z http://www.biopharminternational.com/biopharm/data/articlestandard/biopharm/272009/608689/bp0709_plasmid_DNA_culture_flash.jpg XML