Sponsored Whitepapers

Feb 22, 2018
Sponsored Content
Optimizing purification conditions is an essential step prior to automating multicolumn purification. Save time performing mAb purifications with the NGC Chromatography System to find your ideal conditions in hours or days versus weeks.
Feb 22, 2018
Sponsored Content
TOYOPEARL AF-rProtein A HC-650F is capable of delivering high purity monoclonal antibodies with excellent recovery at loading levels approaching the resin capacity in columns with bed heights as short as 4.2 cm without any increase in aggregate levels or loss of product yield.
Jan 18, 2018
Sponsored Content
Detection and quantification of peptides from transmembrane proteins is important for detection of biomarkers and new protein based drugs. This paper presents a fully automated online digestion and liquid chromatography-mass spectrometry-multiple reaction monitoring (LCMS MRM) analysis of two model transmembrane proteins, a method that improves reproducibility and decreases chances of contamination.
Jan 18, 2018
Sponsored Content
In the pharmaceutical industry, speed to market is essential for gaining a competitive position. New encapsulation technologies now make it possible to streamline research and development activities, improve product development, and jump-start time to market.
Jan 12, 2018
Sponsored Content
In this study, TOYOPEARL® Sulfate-650F, a strong CEX resin was used to capture and remove mAb aggregates and host cell proteins to improve the purity of the mAb eluate post-protein A in a single polishing step.
Dec 14, 2017
Sponsored Content
In this study, TOYOPEARL® Sulfate-650F, a strong CEX resin was used to capture and remove mAb aggregates and host cell proteins to improve the purity of the mAb eluate post-protein A in a single polishing step.
Dec 08, 2017
Sponsored Content
By SCIEX
Routine characterization of biosimilars or batch comparison of manufactured biologic samples is critical to maintain quality for biotherapeutics throughout development and production. The large size and inherent heterogeneity of biologics make these drugs highly complex, requiring robust mass analysis techniques to ensure accuracy in detection of the protein backbone composition as well as the presence of post-translational modifications. This study analyzes trastuzumab biosimilar therapeutics obtained from two different manufacturing sources using subunit characterization to assess therapeutic mass and glycoform abundances. This work demonstrates the use of a fast, reproducible and robust method for biologic subunit analysis on the X500B QTOF system with streamlined and rapid batch processing and product comparison using the BioPharmaView™ software.
Nov 27, 2017
Sponsored Content
Hunky fully automates chemical denaturation experiments from start to finish, making it easy to access the unique stability and aggregation information ΔG provides. The four experimental applications span from low protein sample volume, quick screening experiments with C1/2 and Agg Path to robust stability quantification and aggregation measurements with ΔG and ΔGtrend. Hunky allows researchers to quantitate stability and avoid potential aggregation issues earlier than ever before.
Nov 23, 2017
Sponsored Content
The formation of aggregates is a common occurrence during mAb purification and can happen for several reasons. Generally, the formation of aggregates is shown to be product or process related. Here we show how on-column aggregate formation is dependent on the resin type and on the resin’s interactions with the mAb loaded. Among the various resins tested, a high-resolution cation exchanger, demonstrated the lowest proportion of on-column aggregate formation with two mAbs. On-column aggregate formation can therefore be minimized by proper resin screening and subsequent optimization of resin type used for process development.
Nov 20, 2017
Sponsored Content
Methods for capture of scFV and post-capture-washing condition with chaotropic agents are described. The post-capture-washing condition significantly improves HCP removal to increase scFV purity.
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